Stability and conjugal transfer kinetics of a TOL plasmid in Pseudomonas aeruginosa PAO 1162

Barth F. Smets; Bruce E. Rittmann; David A. Stahl

doi:10.1111/j.1574-6941.1994.tb00256.x

Stability and conjugal transfer kinetics of a TOL plasmid in Pseudomonas aeruginosa PAO 1162

Barth F. Smets, Bruce E. Rittmann, David A. Stahl

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Batch mating experiments were employed to study the kinetics of the conjugal transfer of a TOL plasmid, using the transconjugant strain Pseudomonas aeruginosa PAO 1162 (TOL) as the plasmid donor and Pseudomonas putida PB 2442 and Pseudomonas aeruginosa PAO 1162N as the plasmid recipients. Transfer rates from PAO 1162 (TOL) to PAO 1162N and PB 2442 measured for exponentially grown PAO 1162 (TOL) were 1.81 × 10^-14 (standard error (S.E.) 1.25 × 10^-15) ml·cell^-1min^-1 and 3.32 × 10^-13 (S.E. 4.42 × 10^-14) ml·cell^-1min^-1, respectively. The instability of the TOL plasmid in PAO 1162 (TOL) was evaluated under conditions that were non-selective for maintenance of the TOL catabolic functions. The measured rates of instability were 6.7 10^-6 to 8.3 10^-6 min^-1, and the loss of the catabolic functions was mainly caused by structural instability of the plasmid.

Original language	English (US)
Pages (from-to)	337-350
Number of pages	14
Journal	FEMS microbiology ecology
Volume	15
Issue number	3-4
DOIs	https://doi.org/10.1111/j.1574-6941.1994.tb00256.x
State	Published - Dec 1994
Externally published	Yes

Keywords

Conjugation
Kinetics
Plasmid stability
Pseudomonas aeruginosa
Pseudomonas putida
TOL

ASJC Scopus subject areas

Microbiology
Ecology
Applied Microbiology and Biotechnology

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10.1111/j.1574-6941.1994.tb00256.x

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title = "Stability and conjugal transfer kinetics of a TOL plasmid in Pseudomonas aeruginosa PAO 1162",

abstract = "Batch mating experiments were employed to study the kinetics of the conjugal transfer of a TOL plasmid, using the transconjugant strain Pseudomonas aeruginosa PAO 1162 (TOL) as the plasmid donor and Pseudomonas putida PB 2442 and Pseudomonas aeruginosa PAO 1162N as the plasmid recipients. Transfer rates from PAO 1162 (TOL) to PAO 1162N and PB 2442 measured for exponentially grown PAO 1162 (TOL) were 1.81 × 10-14 (standard error (S.E.) 1.25 × 10-15) ml·cell-1min-1 and 3.32 × 10-13 (S.E. 4.42 × 10-14) ml·cell-1min-1, respectively. The instability of the TOL plasmid in PAO 1162 (TOL) was evaluated under conditions that were non-selective for maintenance of the TOL catabolic functions. The measured rates of instability were 6.7 10-6 to 8.3 10-6 min-1, and the loss of the catabolic functions was mainly caused by structural instability of the plasmid.",

keywords = "Conjugation, Kinetics, Plasmid stability, Pseudomonas aeruginosa, Pseudomonas putida, TOL",

author = "Smets, {Barth F.} and Rittmann, {Bruce E.} and Stahl, {David A.}",

note = "Funding Information: This research was funded in part by the United States Environmental Protection Agency through cooperative agreement CR-812582-03 to the Advanced Environmental Control Technology Research Center. Additional funding was provided by the Amoco Oil Company.",

year = "1994",

month = dec,

doi = "10.1111/j.1574-6941.1994.tb00256.x",

language = "English (US)",

volume = "15",

pages = "337--350",

journal = "FEMS microbiology ecology",

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AU - Smets, Barth F.

AU - Rittmann, Bruce E.

AU - Stahl, David A.

N1 - Funding Information: This research was funded in part by the United States Environmental Protection Agency through cooperative agreement CR-812582-03 to the Advanced Environmental Control Technology Research Center. Additional funding was provided by the Amoco Oil Company.

PY - 1994/12

Y1 - 1994/12

N2 - Batch mating experiments were employed to study the kinetics of the conjugal transfer of a TOL plasmid, using the transconjugant strain Pseudomonas aeruginosa PAO 1162 (TOL) as the plasmid donor and Pseudomonas putida PB 2442 and Pseudomonas aeruginosa PAO 1162N as the plasmid recipients. Transfer rates from PAO 1162 (TOL) to PAO 1162N and PB 2442 measured for exponentially grown PAO 1162 (TOL) were 1.81 × 10-14 (standard error (S.E.) 1.25 × 10-15) ml·cell-1min-1 and 3.32 × 10-13 (S.E. 4.42 × 10-14) ml·cell-1min-1, respectively. The instability of the TOL plasmid in PAO 1162 (TOL) was evaluated under conditions that were non-selective for maintenance of the TOL catabolic functions. The measured rates of instability were 6.7 10-6 to 8.3 10-6 min-1, and the loss of the catabolic functions was mainly caused by structural instability of the plasmid.

AB - Batch mating experiments were employed to study the kinetics of the conjugal transfer of a TOL plasmid, using the transconjugant strain Pseudomonas aeruginosa PAO 1162 (TOL) as the plasmid donor and Pseudomonas putida PB 2442 and Pseudomonas aeruginosa PAO 1162N as the plasmid recipients. Transfer rates from PAO 1162 (TOL) to PAO 1162N and PB 2442 measured for exponentially grown PAO 1162 (TOL) were 1.81 × 10-14 (standard error (S.E.) 1.25 × 10-15) ml·cell-1min-1 and 3.32 × 10-13 (S.E. 4.42 × 10-14) ml·cell-1min-1, respectively. The instability of the TOL plasmid in PAO 1162 (TOL) was evaluated under conditions that were non-selective for maintenance of the TOL catabolic functions. The measured rates of instability were 6.7 10-6 to 8.3 10-6 min-1, and the loss of the catabolic functions was mainly caused by structural instability of the plasmid.

KW - Conjugation

KW - Kinetics

KW - Plasmid stability

KW - Pseudomonas aeruginosa

KW - Pseudomonas putida

KW - TOL

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Stability and conjugal transfer kinetics of a TOL plasmid in Pseudomonas aeruginosa PAO 1162

Abstract

Keywords

ASJC Scopus subject areas

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