Stability and conjugal transfer kinetics of a TOL plasmid in Pseudomonas aeruginosa PAO 1162

Barth F. Smets, Bruce E. Rittmann, David A. Stahl

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Batch mating experiments were employed to study the kinetics of the conjugal transfer of a TOL plasmid, using the transconjugant strain Pseudomonas aeruginosa PAO 1162 (TOL) as the plasmid donor and Pseudomonas putida PB 2442 and Pseudomonas aeruginosa PAO 1162N as the plasmid recipients. Transfer rates from PAO 1162 (TOL) to PAO 1162N and PB 2442 measured for exponentially grown PAO 1162 (TOL) were 1.81 × 10-14 (standard error (S.E.) 1.25 × 10-15) ml·cell-1min-1 and 3.32 × 10-13 (S.E. 4.42 × 10-14) ml·cell-1min-1, respectively. The instability of the TOL plasmid in PAO 1162 (TOL) was evaluated under conditions that were non-selective for maintenance of the TOL catabolic functions. The measured rates of instability were 6.7 10-6 to 8.3 10-6 min-1, and the loss of the catabolic functions was mainly caused by structural instability of the plasmid.

Original languageEnglish (US)
Pages (from-to)337-350
Number of pages14
JournalFEMS microbiology ecology
Volume15
Issue number3-4
DOIs
StatePublished - Dec 1994
Externally publishedYes

Keywords

  • Conjugation
  • Kinetics
  • Plasmid stability
  • Pseudomonas aeruginosa
  • Pseudomonas putida
  • TOL

ASJC Scopus subject areas

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

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