Spontaneous mutational variation for body size in Caenorhabditis elegans

Ricardo B.R. Azevedo, Peter D. Keightley, Camilla Laurén-Määttä, Larissa L. Vassilieva, Michael Lynch, Armand M. Leroi

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We measured the impact of new mutations on genetic variation for body size in two independent sets of C. elegans spontaneous mutation-accumulation (MA) lines, derived from the N2 strain, that had been maintained by selfing for 60 or 152 generations. The two sets of lines gave broadly consistent results. The change of among-line genetic variation between cryopreserved controls and the MA lines implied that broad sense heritability increased by 0.4% per generation. Overall, MA reduced mean body size by ∼0.1% per generation. The genome-wide rate for mutations with detectable effects on size was estimated to be ∼0.0025 per haploid genome per generation, and their mean effects were ∼20%. The proportion of mutations that increase body size was estimated by maximum likelihood to be no more than 20%, suggesting that the amount of mutational variation available for selection for increased size could be quite small. This hypothesis was supported by an artificial selection experiment on adult body size, started from a single highly inbred N2 individual. We observed a strongly asymmetrical response to selection of a magnitude consistent with the input of mutational variance observed in the MA experiment.

Original languageEnglish (US)
Pages (from-to)755-765
Number of pages11
JournalGenetics
Volume162
Issue number2
StatePublished - Oct 1 2002
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Body Size
Genome
Mutation
Haploidy
Mutation Rate
Mutation Accumulation

ASJC Scopus subject areas

  • Genetics

Cite this

Azevedo, R. B. R., Keightley, P. D., Laurén-Määttä, C., Vassilieva, L. L., Lynch, M., & Leroi, A. M. (2002). Spontaneous mutational variation for body size in Caenorhabditis elegans. Genetics, 162(2), 755-765.

Spontaneous mutational variation for body size in Caenorhabditis elegans. / Azevedo, Ricardo B.R.; Keightley, Peter D.; Laurén-Määttä, Camilla; Vassilieva, Larissa L.; Lynch, Michael; Leroi, Armand M.

In: Genetics, Vol. 162, No. 2, 01.10.2002, p. 755-765.

Research output: Contribution to journalArticle

Azevedo, RBR, Keightley, PD, Laurén-Määttä, C, Vassilieva, LL, Lynch, M & Leroi, AM 2002, 'Spontaneous mutational variation for body size in Caenorhabditis elegans', Genetics, vol. 162, no. 2, pp. 755-765.
Azevedo RBR, Keightley PD, Laurén-Määttä C, Vassilieva LL, Lynch M, Leroi AM. Spontaneous mutational variation for body size in Caenorhabditis elegans. Genetics. 2002 Oct 1;162(2):755-765.
Azevedo, Ricardo B.R. ; Keightley, Peter D. ; Laurén-Määttä, Camilla ; Vassilieva, Larissa L. ; Lynch, Michael ; Leroi, Armand M. / Spontaneous mutational variation for body size in Caenorhabditis elegans. In: Genetics. 2002 ; Vol. 162, No. 2. pp. 755-765.
@article{4fba3a7cb0ac44cf97677489a4e92155,
title = "Spontaneous mutational variation for body size in Caenorhabditis elegans",
abstract = "We measured the impact of new mutations on genetic variation for body size in two independent sets of C. elegans spontaneous mutation-accumulation (MA) lines, derived from the N2 strain, that had been maintained by selfing for 60 or 152 generations. The two sets of lines gave broadly consistent results. The change of among-line genetic variation between cryopreserved controls and the MA lines implied that broad sense heritability increased by 0.4{\%} per generation. Overall, MA reduced mean body size by ∼0.1{\%} per generation. The genome-wide rate for mutations with detectable effects on size was estimated to be ∼0.0025 per haploid genome per generation, and their mean effects were ∼20{\%}. The proportion of mutations that increase body size was estimated by maximum likelihood to be no more than 20{\%}, suggesting that the amount of mutational variation available for selection for increased size could be quite small. This hypothesis was supported by an artificial selection experiment on adult body size, started from a single highly inbred N2 individual. We observed a strongly asymmetrical response to selection of a magnitude consistent with the input of mutational variance observed in the MA experiment.",
author = "Azevedo, {Ricardo B.R.} and Keightley, {Peter D.} and Camilla Laur{\'e}n-M{\"a}{\"a}tt{\"a} and Vassilieva, {Larissa L.} and Michael Lynch and Leroi, {Armand M.}",
year = "2002",
month = "10",
day = "1",
language = "English (US)",
volume = "162",
pages = "755--765",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "2",

}

TY - JOUR

T1 - Spontaneous mutational variation for body size in Caenorhabditis elegans

AU - Azevedo, Ricardo B.R.

AU - Keightley, Peter D.

AU - Laurén-Määttä, Camilla

AU - Vassilieva, Larissa L.

AU - Lynch, Michael

AU - Leroi, Armand M.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - We measured the impact of new mutations on genetic variation for body size in two independent sets of C. elegans spontaneous mutation-accumulation (MA) lines, derived from the N2 strain, that had been maintained by selfing for 60 or 152 generations. The two sets of lines gave broadly consistent results. The change of among-line genetic variation between cryopreserved controls and the MA lines implied that broad sense heritability increased by 0.4% per generation. Overall, MA reduced mean body size by ∼0.1% per generation. The genome-wide rate for mutations with detectable effects on size was estimated to be ∼0.0025 per haploid genome per generation, and their mean effects were ∼20%. The proportion of mutations that increase body size was estimated by maximum likelihood to be no more than 20%, suggesting that the amount of mutational variation available for selection for increased size could be quite small. This hypothesis was supported by an artificial selection experiment on adult body size, started from a single highly inbred N2 individual. We observed a strongly asymmetrical response to selection of a magnitude consistent with the input of mutational variance observed in the MA experiment.

AB - We measured the impact of new mutations on genetic variation for body size in two independent sets of C. elegans spontaneous mutation-accumulation (MA) lines, derived from the N2 strain, that had been maintained by selfing for 60 or 152 generations. The two sets of lines gave broadly consistent results. The change of among-line genetic variation between cryopreserved controls and the MA lines implied that broad sense heritability increased by 0.4% per generation. Overall, MA reduced mean body size by ∼0.1% per generation. The genome-wide rate for mutations with detectable effects on size was estimated to be ∼0.0025 per haploid genome per generation, and their mean effects were ∼20%. The proportion of mutations that increase body size was estimated by maximum likelihood to be no more than 20%, suggesting that the amount of mutational variation available for selection for increased size could be quite small. This hypothesis was supported by an artificial selection experiment on adult body size, started from a single highly inbred N2 individual. We observed a strongly asymmetrical response to selection of a magnitude consistent with the input of mutational variance observed in the MA experiment.

UR - http://www.scopus.com/inward/record.url?scp=0036811121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036811121&partnerID=8YFLogxK

M3 - Article

C2 - 12399386

AN - SCOPUS:0036811121

VL - 162

SP - 755

EP - 765

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 2

ER -