Spectroscopic characterization of PS I core complexes from thermophilic Synechococcus sp. Identical reoxidation kinetics of A-1 before and after removal of theiron-sulfur-clusters FA and FB

Jürgen Lüneberg, Petra Fromme, Petra Jekow, Eberhard Schlodder

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Monomeric and trimeric PS I complexes missing the three stromal subunits E,C and D (termed PS I core complexes) were prepared from the thermophilic cyanobacterium Synechococcus sp. by incubation with urea. The subunits E,C and D are sequentially removed. In the monomeric PS I the subunit C is removed with a half life of approx. 5 min. This is about eight times faster than in the trimeric PS I complex. In parallel with the removal of the F A B containing subunit C the reduction kinetics of P700+ changed from a half life of about 25 ms to about 750 μs. The partner of P700+ in the 750 μs charge recombination was identified to be FX by the difference spectrum of this phase. There are some minor differences in the spectra of trimeric and monomeric PS I core complexes. At 77K the forward electron transfer from A -1 to FX is blocked in the major fraction of the PS I core complexes and P700+A-1 recombines with a half life of about 220 μs. In the remaining fraction P700+FX- is formed and decays with a half life of approx. 10 ms at 77 K. The kinetics of the forward electron transfer from A-1 to the iron-sulfur-clusters was measured in the native PS I and the corresponding core complexes. The reoxidation kinetics of A-1 are identical in both cases (t 1 2 = 180 ns). We conclude that Fx is an obligatory intermediate in the normal forward electron transfer.

Original languageEnglish (US)
Pages (from-to)197-202
Number of pages6
JournalFEBS Letters
Volume338
Issue number2
DOIs
StatePublished - Jan 31 1994
Externally publishedYes

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Keywords

  • Absorption difference spectroscopy
  • Core protein
  • Electron transfer
  • Iron sulfur center
  • Photosystem I

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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