Specificity of Deoxyribonucleic Acid Cleavage by Bleomycin, Phleomycin, and Tallysomycin

Joseph Kross, W. David Henner, William A. Haseltine, Sidney M. Hecht

Research output: Contribution to journalArticle

126 Scopus citations

Abstract

The sites of cleavage of DNA by bleomycin A2, bleomycin B2, phleomycin, tallysomycin A, and Blenoxane (Bristol-Meyers) in reactions containing equimolar Fe2+ and atmospheric oxygen were analyzed by gel electrophoresis of 32P end labeled DNA fragments. Bleomycin A2 and bleomycin B2 reactions cleaved DNA at all sites with a frequency equal to that of Blenoxane. At high concentrations of bleomycin the site specificity of cleavage was unchanged. Bleomycin cleavage sites and phleomycin cleavage sites are a subset of sites cleaved in reactions containing tallysomycin A. The nature of 5’ and 3’ termini induced by bleomycin cleavage was investigated. Electrophoresis of bleomycin-induced fragments after alkaline phosphatase or polynucleotide kinase treatment indicated that 5’ termini are phosphoryl groups but 3’ termini are not simple phosphoryl groups. Analysis of bleomycin cleavage of single-stranded DNA substrate showed that cleavage occurs only in regions of potentially double-stranded looped-back sequences. Possible mechanisms for determination of bleomycin cleavage sequence specificity are discussed.

Original languageEnglish (US)
Pages (from-to)4310-4318
Number of pages9
JournalBiochemistry
Volume21
Issue number18
DOIs
StatePublished - Aug 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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