Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin, and tallysomycin

Joseph Kross, W. David Henner, Sidney M. Hecht, Sidney Hecht

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

The sites of cleavage of DNA by bleomycin A2, bleomycin B2, phleomycin, tallysomycin A, and Blenoxane (Bristol-Meyers) in reactions containing equimolar Fe2+ and atmospheric oxygen were analyzed by gel electrophoresis of 32P end labeled DNA fragments. Bleomycin A2 and bleomycin B2 reactions cleaved DNA at all sites with a frequency equal to that of Blenoxane. At high concentrations of bleomycin the site specificity of cleavage was unchanged. Bleomycin cleavage sites and phleomycin cleavage sites are a subset of sites cleaved in reactions containing tallysomycin A. The nature of 5′ and 3′ termini induced by bleomycin cleavage was investigated. Electrophoresis of bleomycin-induced fragments after alkaline phosphatase or polynucleotide kinase treatment indicated that 5′ termini are phosphoryl groups but 3′ termini are not simple phosphoryl groups. Analysis of bleomycin cleavage of single-stranded DNA substrate showed that cleavage occurs only in regions of potentially double-stranded looped-back sequences. Possible mechanisms for determination of bleomycin cleavage sequence specificity are discussed.

Original languageEnglish (US)
Pages (from-to)4310-4318
Number of pages9
JournalBiochemistry
Volume21
Issue number18
StatePublished - 1982
Externally publishedYes

Fingerprint

Phleomycins
Bleomycin
DNA
Electrophoresis
talisomycin
Polynucleotide 5'-Hydroxyl-Kinase
DNA Cleavage
Single-Stranded DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin, and tallysomycin. / Kross, Joseph; Henner, W. David; Hecht, Sidney M.; Hecht, Sidney.

In: Biochemistry, Vol. 21, No. 18, 1982, p. 4310-4318.

Research output: Contribution to journalArticle

Kross, J, Henner, WD, Hecht, SM & Hecht, S 1982, 'Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin, and tallysomycin', Biochemistry, vol. 21, no. 18, pp. 4310-4318.
Kross, Joseph ; Henner, W. David ; Hecht, Sidney M. ; Hecht, Sidney. / Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin, and tallysomycin. In: Biochemistry. 1982 ; Vol. 21, No. 18. pp. 4310-4318.
@article{42ece88f171e438db94b00d608224318,
title = "Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin, and tallysomycin",
abstract = "The sites of cleavage of DNA by bleomycin A2, bleomycin B2, phleomycin, tallysomycin A, and Blenoxane (Bristol-Meyers) in reactions containing equimolar Fe2+ and atmospheric oxygen were analyzed by gel electrophoresis of 32P end labeled DNA fragments. Bleomycin A2 and bleomycin B2 reactions cleaved DNA at all sites with a frequency equal to that of Blenoxane. At high concentrations of bleomycin the site specificity of cleavage was unchanged. Bleomycin cleavage sites and phleomycin cleavage sites are a subset of sites cleaved in reactions containing tallysomycin A. The nature of 5′ and 3′ termini induced by bleomycin cleavage was investigated. Electrophoresis of bleomycin-induced fragments after alkaline phosphatase or polynucleotide kinase treatment indicated that 5′ termini are phosphoryl groups but 3′ termini are not simple phosphoryl groups. Analysis of bleomycin cleavage of single-stranded DNA substrate showed that cleavage occurs only in regions of potentially double-stranded looped-back sequences. Possible mechanisms for determination of bleomycin cleavage sequence specificity are discussed.",
author = "Joseph Kross and Henner, {W. David} and Hecht, {Sidney M.} and Sidney Hecht",
year = "1982",
language = "English (US)",
volume = "21",
pages = "4310--4318",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "18",

}

TY - JOUR

T1 - Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin, and tallysomycin

AU - Kross, Joseph

AU - Henner, W. David

AU - Hecht, Sidney M.

AU - Hecht, Sidney

PY - 1982

Y1 - 1982

N2 - The sites of cleavage of DNA by bleomycin A2, bleomycin B2, phleomycin, tallysomycin A, and Blenoxane (Bristol-Meyers) in reactions containing equimolar Fe2+ and atmospheric oxygen were analyzed by gel electrophoresis of 32P end labeled DNA fragments. Bleomycin A2 and bleomycin B2 reactions cleaved DNA at all sites with a frequency equal to that of Blenoxane. At high concentrations of bleomycin the site specificity of cleavage was unchanged. Bleomycin cleavage sites and phleomycin cleavage sites are a subset of sites cleaved in reactions containing tallysomycin A. The nature of 5′ and 3′ termini induced by bleomycin cleavage was investigated. Electrophoresis of bleomycin-induced fragments after alkaline phosphatase or polynucleotide kinase treatment indicated that 5′ termini are phosphoryl groups but 3′ termini are not simple phosphoryl groups. Analysis of bleomycin cleavage of single-stranded DNA substrate showed that cleavage occurs only in regions of potentially double-stranded looped-back sequences. Possible mechanisms for determination of bleomycin cleavage sequence specificity are discussed.

AB - The sites of cleavage of DNA by bleomycin A2, bleomycin B2, phleomycin, tallysomycin A, and Blenoxane (Bristol-Meyers) in reactions containing equimolar Fe2+ and atmospheric oxygen were analyzed by gel electrophoresis of 32P end labeled DNA fragments. Bleomycin A2 and bleomycin B2 reactions cleaved DNA at all sites with a frequency equal to that of Blenoxane. At high concentrations of bleomycin the site specificity of cleavage was unchanged. Bleomycin cleavage sites and phleomycin cleavage sites are a subset of sites cleaved in reactions containing tallysomycin A. The nature of 5′ and 3′ termini induced by bleomycin cleavage was investigated. Electrophoresis of bleomycin-induced fragments after alkaline phosphatase or polynucleotide kinase treatment indicated that 5′ termini are phosphoryl groups but 3′ termini are not simple phosphoryl groups. Analysis of bleomycin cleavage of single-stranded DNA substrate showed that cleavage occurs only in regions of potentially double-stranded looped-back sequences. Possible mechanisms for determination of bleomycin cleavage sequence specificity are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0020365691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020365691&partnerID=8YFLogxK

M3 - Article

VL - 21

SP - 4310

EP - 4318

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 18

ER -