Specificity and mobility of biomacromolecular, multivalent constructs for cellular targeting

Elena V. Rosca, Jill M. Stukel, Robert J. Gillies, Josef Vagner, Michael Caplan

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Effective targeting of drugs to cells requires that the drug reach the target cell and interact specifically with it. In this study, we synthesized a biomacromolecular, multivalent construct intended to target glioblastoma tumors. The construct was created by linking three dodecapeptides, reported to bind the α6β 1 integrin, with poly(ethylene glycol) linkers. The construct is intended to be delivered locally, and it demonstrates a more homogeneous and more rapid perfusion profile in comparison with quantum dots. The binding specificity of the construct was investigated by using glioblastoma cells and normal human astrocyte cells. The results reveal qualitative differences in binding between glioma and normal human astrocyte cells, with a moderate increase in binding avidity due to multivalency (0.79 μM for the trivalent construct versus 4.28 μM for the dodecapeptide). Overall, biomacromolecular constructs appear to be a promising approach for targeting with high biocompatibility, good perfusion abilities, and specificity.

Original languageEnglish (US)
Pages (from-to)3830-3835
Number of pages6
JournalBiomacromolecules
Volume8
Issue number12
DOIs
StatePublished - Dec 2007

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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    Rosca, E. V., Stukel, J. M., Gillies, R. J., Vagner, J., & Caplan, M. (2007). Specificity and mobility of biomacromolecular, multivalent constructs for cellular targeting. Biomacromolecules, 8(12), 3830-3835. https://doi.org/10.1021/bm700791a