Species Specificity of Ectromelia Virus and Vaccinia Virus Interferon-γ Binding Proteins

K. Mossman, C. Upton, R. M.L. Buller, Douglas McFadden

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Interferon-γ functions within the immune system as a potent anti-viral and immunoregulatory cytokine. In order to successfully replicate within a host cell, poxviruses have evolved a number of strategies to counteract the pleiotropic effects of interferon-γ. In particular, the leporipoxvirus myxoma virus was shown to express an extracellular soluble interferon-γ receptor homolog, denoted M-T7, which is capable of inhibiting the anti-viral activities of rabbit interferon-γ (C. Upton, K. Mossman, and G. McFadden, 1992, Science 258, 1369-1372). Here, we demonstrate that expression of soluble interferonγ receptor homologs appears to be characteristic of all poxviruses tested, including Shope fibroma virus, vaccinia virus (strains WR and IHDW), ectromelia virus, cowpox virus, and rabbitpox virus. We have cloned, sequenced, and characterized the interferon-γ binding protein in supernatants from ectromelia virus-infected cells, and demonstrate the capability of this soluble protein to bind human, murine, and rabbit interferon-γ with similar affinity. We also investigate the properties of the vaccinia virus interferon-γ binding protein and demonstrate that this protein binds human and rabbit interferon-γ with similar affinity and binds murine interferon-γ with a significantly lower relative affinity. The implications of these studies with respect to viral pathogenesis and the evolutionary relationship between a virus and its host are discussed.

Original languageEnglish (US)
Article number71208
Pages (from-to)762-769
Number of pages8
JournalVirology
Volume208
Issue number2
DOIs
StatePublished - Apr 20 1995
Externally publishedYes

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Ectromelia virus
Species Specificity
Virus Attachment
Vaccinia virus
Interferons
Carrier Proteins
Interferon Receptors
Poxviridae
Rabbits
Leporipoxvirus
Rabbit Fibroma Virus
Myxoma virus
Cowpox virus
Immune System
Proteins
Cytokines
Viruses

ASJC Scopus subject areas

  • Virology

Cite this

Species Specificity of Ectromelia Virus and Vaccinia Virus Interferon-γ Binding Proteins. / Mossman, K.; Upton, C.; Buller, R. M.L.; McFadden, Douglas.

In: Virology, Vol. 208, No. 2, 71208, 20.04.1995, p. 762-769.

Research output: Contribution to journalArticle

Mossman, K. ; Upton, C. ; Buller, R. M.L. ; McFadden, Douglas. / Species Specificity of Ectromelia Virus and Vaccinia Virus Interferon-γ Binding Proteins. In: Virology. 1995 ; Vol. 208, No. 2. pp. 762-769.
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