Species-diagnostic single-nucleotide polymorphism and sequence-tagged site markers for the parasitic wasp genus Nasonia (Hymenoptera: Pteromalidae)

O. Niehuis, A. K. Judson, J. H. Werren, W. B. Hunter, P. M. Dang, S. E. Dowd, B. Grillenberger, L. W. Beukeboom, Juergen Gadau

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Wasps of the genus Nasonia are important biological control agents of house flies and related filth flies, which are major vectors of human pathogens. Species of Nasonia (Hymenoptera: Pteromalidae) are not easily differentiated from one another by morphological characters, and molecular markers for their reliable identification have been missing so far. Here, we report eight single-nucleotide polymorphism and three sequence-tagged site markers derived from expressed sequenced tag libraries for the two closely related and regionally sympatric species N. giraulti and N. vitripennis. We studied variation of these markers in natural populations of the two species, and we mapped them in the Nasonia genome. The markers are species-diagnostic and evenly spread over all five chromosomes. They are ideal for rapid species identification and hybrid recognition, and they can be used to map economically relevant quantitative trait loci in the Nasonia genome.

Original languageEnglish (US)
Pages (from-to)1033-1036
Number of pages4
JournalJournal of Economic Entomology
Volume100
Issue number4
DOIs
StatePublished - Aug 2007

Fingerprint

Nasonia
Pteromalidae
wasp
single nucleotide polymorphism
polymorphism
Hymenoptera
genome
Musca domestica
sympatry
biological control agents
quantitative trait loci
biological control
chromosome
chromosomes
pathogen
genetic markers
parasitic wasps
marker
pathogens

Keywords

  • Ecotilling
  • Genome mapping
  • Nasonia
  • Species identification

ASJC Scopus subject areas

  • Insect Science

Cite this

Species-diagnostic single-nucleotide polymorphism and sequence-tagged site markers for the parasitic wasp genus Nasonia (Hymenoptera : Pteromalidae). / Niehuis, O.; Judson, A. K.; Werren, J. H.; Hunter, W. B.; Dang, P. M.; Dowd, S. E.; Grillenberger, B.; Beukeboom, L. W.; Gadau, Juergen.

In: Journal of Economic Entomology, Vol. 100, No. 4, 08.2007, p. 1033-1036.

Research output: Contribution to journalArticle

Niehuis, O. ; Judson, A. K. ; Werren, J. H. ; Hunter, W. B. ; Dang, P. M. ; Dowd, S. E. ; Grillenberger, B. ; Beukeboom, L. W. ; Gadau, Juergen. / Species-diagnostic single-nucleotide polymorphism and sequence-tagged site markers for the parasitic wasp genus Nasonia (Hymenoptera : Pteromalidae). In: Journal of Economic Entomology. 2007 ; Vol. 100, No. 4. pp. 1033-1036.
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AU - Werren, J. H.

AU - Hunter, W. B.

AU - Dang, P. M.

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