Species-diagnostic single-nucleotide polymorphism and sequence-tagged site markers for the parasitic wasp genus Nasonia (Hymenoptera: Pteromalidae)

O. Niehuis; A. K. Judson; J. H. Werren; W. B. Hunter; P. M. Dang; S. E. Dowd; B. Grillenberger; L. W. Beukeboom; Juergen Gadau

doi:10.1603/0022-0493(2007)100[1033:SSPASS]2.0.CO;2

Species-diagnostic single-nucleotide polymorphism and sequence-tagged site markers for the parasitic wasp genus Nasonia (Hymenoptera: Pteromalidae)

O. Niehuis, A. K. Judson, J. H. Werren, W. B. Hunter, P. M. Dang, S. E. Dowd, B. Grillenberger, L. W. Beukeboom, Juergen Gadau

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Wasps of the genus Nasonia are important biological control agents of house flies and related filth flies, which are major vectors of human pathogens. Species of Nasonia (Hymenoptera: Pteromalidae) are not easily differentiated from one another by morphological characters, and molecular markers for their reliable identification have been missing so far. Here, we report eight single-nucleotide polymorphism and three sequence-tagged site markers derived from expressed sequenced tag libraries for the two closely related and regionally sympatric species N. giraulti and N. vitripennis. We studied variation of these markers in natural populations of the two species, and we mapped them in the Nasonia genome. The markers are species-diagnostic and evenly spread over all five chromosomes. They are ideal for rapid species identification and hybrid recognition, and they can be used to map economically relevant quantitative trait loci in the Nasonia genome.

Original language	English (US)
Pages (from-to)	1033-1036
Number of pages	4
Journal	Journal of Economic Entomology
Volume	100
Issue number	4
DOIs	https://doi.org/10.1603/0022-0493(2007)100[1033:SSPASS]2.0.CO;2
State	Published - Aug 2007

Keywords

Ecotilling
Genome mapping
Nasonia
Species identification

ASJC Scopus subject areas

Ecology
Insect Science

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10.1603/0022-0493(2007)100[1033:SSPASS]2.0.CO;2

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Niehuis, O., Judson, A. K., Werren, J. H., Hunter, W. B., Dang, P. M., Dowd, S. E., Grillenberger, B., Beukeboom, L. W., & Gadau, J. (2007). Species-diagnostic single-nucleotide polymorphism and sequence-tagged site markers for the parasitic wasp genus Nasonia (Hymenoptera: Pteromalidae). Journal of Economic Entomology, 100(4), 1033-1036. https://doi.org/10.1603/0022-0493(2007)100[1033:SSPASS]2.0.CO;2

Niehuis, O, Judson, AK, Werren, JH, Hunter, WB, Dang, PM, Dowd, SE, Grillenberger, B, Beukeboom, LW & Gadau, J 2007, 'Species-diagnostic single-nucleotide polymorphism and sequence-tagged site markers for the parasitic wasp genus Nasonia (Hymenoptera: Pteromalidae)', Journal of Economic Entomology, vol. 100, no. 4, pp. 1033-1036. https://doi.org/10.1603/0022-0493(2007)100[1033:SSPASS]2.0.CO;2

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title = "Species-diagnostic single-nucleotide polymorphism and sequence-tagged site markers for the parasitic wasp genus Nasonia (Hymenoptera: Pteromalidae)",

abstract = "Wasps of the genus Nasonia are important biological control agents of house flies and related filth flies, which are major vectors of human pathogens. Species of Nasonia (Hymenoptera: Pteromalidae) are not easily differentiated from one another by morphological characters, and molecular markers for their reliable identification have been missing so far. Here, we report eight single-nucleotide polymorphism and three sequence-tagged site markers derived from expressed sequenced tag libraries for the two closely related and regionally sympatric species N. giraulti and N. vitripennis. We studied variation of these markers in natural populations of the two species, and we mapped them in the Nasonia genome. The markers are species-diagnostic and evenly spread over all five chromosomes. They are ideal for rapid species identification and hybrid recognition, and they can be used to map economically relevant quantitative trait loci in the Nasonia genome.",

keywords = "Ecotilling, Genome mapping, Nasonia, Species identification",

author = "O. Niehuis and Judson, {A. K.} and Werren, {J. H.} and Hunter, {W. B.} and Dang, {P. M.} and Dowd, {S. E.} and B. Grillenberger and Beukeboom, {L. W.} and Juergen Gadau",

year = "2007",

month = aug,

doi = "10.1603/0022-0493(2007)100[1033:SSPASS]2.0.CO;2",

language = "English (US)",

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AU - Niehuis, O.

AU - Judson, A. K.

AU - Werren, J. H.

AU - Hunter, W. B.

AU - Dang, P. M.

AU - Dowd, S. E.

AU - Grillenberger, B.

AU - Beukeboom, L. W.

AU - Gadau, Juergen

PY - 2007/8

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N2 - Wasps of the genus Nasonia are important biological control agents of house flies and related filth flies, which are major vectors of human pathogens. Species of Nasonia (Hymenoptera: Pteromalidae) are not easily differentiated from one another by morphological characters, and molecular markers for their reliable identification have been missing so far. Here, we report eight single-nucleotide polymorphism and three sequence-tagged site markers derived from expressed sequenced tag libraries for the two closely related and regionally sympatric species N. giraulti and N. vitripennis. We studied variation of these markers in natural populations of the two species, and we mapped them in the Nasonia genome. The markers are species-diagnostic and evenly spread over all five chromosomes. They are ideal for rapid species identification and hybrid recognition, and they can be used to map economically relevant quantitative trait loci in the Nasonia genome.

AB - Wasps of the genus Nasonia are important biological control agents of house flies and related filth flies, which are major vectors of human pathogens. Species of Nasonia (Hymenoptera: Pteromalidae) are not easily differentiated from one another by morphological characters, and molecular markers for their reliable identification have been missing so far. Here, we report eight single-nucleotide polymorphism and three sequence-tagged site markers derived from expressed sequenced tag libraries for the two closely related and regionally sympatric species N. giraulti and N. vitripennis. We studied variation of these markers in natural populations of the two species, and we mapped them in the Nasonia genome. The markers are species-diagnostic and evenly spread over all five chromosomes. They are ideal for rapid species identification and hybrid recognition, and they can be used to map economically relevant quantitative trait loci in the Nasonia genome.

KW - Ecotilling

KW - Genome mapping

KW - Nasonia

KW - Species identification

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Species-diagnostic single-nucleotide polymorphism and sequence-tagged site markers for the parasitic wasp genus Nasonia (Hymenoptera: Pteromalidae)

Abstract

Keywords

ASJC Scopus subject areas

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