Spatio-temporally controlled transfection of nucleic acid payloads in cell-culture

Tilak Jain, Jit Muthuswamy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

There is a critical need in emerging areas of high-throughput screening of large molecule libraries during drug development, to transfect multiple molecules precisely into cells in a specific location within a culture. In this study, we report the novel use of a microelectrode array technology to achieve spatio-temporally controlled transfection of siRNA molecules into fibroblasts using the mechanism of electroporation. Optimal electroporatian was achieved using cathodic voltage pulses of approximately 4 V and 10 ms duration, with transfection efficiency of approximately 57% and cell viability of 60%. Further development of this technology for gene and RNAi transfection is envisioned to be useful to make significant advances in the field neurogenomics and high-throughput screening.

Original languageEnglish (US)
Title of host publicationProceedings of the 3rd International IEEE EMBS Conference on Neural Engineering
Pages284-286
Number of pages3
DOIs
StatePublished - Sep 25 2007
Externally publishedYes
Event3rd International IEEE EMBS Conference on Neural Engineering - Kohala Coast, HI, United States
Duration: May 2 2007May 5 2007

Publication series

NameProceedings of the 3rd International IEEE EMBS Conference on Neural Engineering

Other

Other3rd International IEEE EMBS Conference on Neural Engineering
CountryUnited States
CityKohala Coast, HI
Period5/2/075/5/07

Keywords

  • Gene delivery
  • Microelectrode arrays
  • RNA
  • siRNA

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Neuroscience (miscellaneous)

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  • Cite this

    Jain, T., & Muthuswamy, J. (2007). Spatio-temporally controlled transfection of nucleic acid payloads in cell-culture. In Proceedings of the 3rd International IEEE EMBS Conference on Neural Engineering (pp. 284-286). [4227271] (Proceedings of the 3rd International IEEE EMBS Conference on Neural Engineering). https://doi.org/10.1109/CNE.2007.369666