@inproceedings{8daabdd122804ccabb18bf84d1c629dd,
title = "Spatio-temporally controlled transfection of nucleic acid payloads in cell-culture",
abstract = "There is a critical need in emerging areas of high-throughput screening of large molecule libraries during drug development, to transfect multiple molecules precisely into cells in a specific location within a culture. In this study, we report the novel use of a microelectrode array technology to achieve spatio-temporally controlled transfection of siRNA molecules into fibroblasts using the mechanism of electroporation. Optimal electroporatian was achieved using cathodic voltage pulses of approximately 4 V and 10 ms duration, with transfection efficiency of approximately 57% and cell viability of 60%. Further development of this technology for gene and RNAi transfection is envisioned to be useful to make significant advances in the field neurogenomics and high-throughput screening.",
keywords = "Gene delivery, Microelectrode arrays, RNA, siRNA",
author = "Tilak Jain and Jit Muthuswamy",
year = "2007",
month = sep,
day = "25",
doi = "10.1109/CNE.2007.369666",
language = "English (US)",
isbn = "1424407923",
series = "Proceedings of the 3rd International IEEE EMBS Conference on Neural Engineering",
pages = "284--286",
booktitle = "Proceedings of the 3rd International IEEE EMBS Conference on Neural Engineering",
note = "3rd International IEEE EMBS Conference on Neural Engineering ; Conference date: 02-05-2007 Through 05-05-2007",
}