Spatial reorganization of actin, tubulin and histone mRNAs during meiotic maturation and fertilization in Xenopus oocytes

Brian A. Perry, David Capco

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The distribution of actin, tubulin and histone mRNAs is examined in full grown oocytes, meiotically mature eggs, and unicellular zygotes. For this analysis, oocytes, eggs and embryos were spatially divided into peripheral and central regions of both the animal and vegetal hemispheres, and the relative amounts and concentrations of these RNAs in each region were then determined. The concentration of actin and tubulin mRNAs is greatest in the periphery, whereas histone mRNA exhibits a uniform concentration throughout the oocyte. In the meiotically mature egg, actin mRNA is still concentrated in the periphery and histone mRNA still exhibits a relatively uniform concentration, but the tubulin mRNAs are more concentrated in the central regions. Following fertilization, however, the greatest concentration of mRNAs for actin, histone and tubulin is in the periphery of the zygote. The results demonstrate the existence of a system capable of altering the distributions of these mRNAs as well as a system which distinguishes between different types of mRNA.

Original languageEnglish (US)
Pages (from-to)99-108
Number of pages10
JournalCell Differentiation and Development
Volume25
Issue number2
DOIs
StatePublished - 1988

Fingerprint

Tubulin
Xenopus
Fertilization
Histones
Oocytes
Actins
Messenger RNA
Zygote
Eggs
Ovum
Embryonic Structures
RNA

Keywords

  • Cortex
  • Egg
  • mRNA
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology

Cite this

@article{fedb6fbb8c824660bba90cef21a33063,
title = "Spatial reorganization of actin, tubulin and histone mRNAs during meiotic maturation and fertilization in Xenopus oocytes",
abstract = "The distribution of actin, tubulin and histone mRNAs is examined in full grown oocytes, meiotically mature eggs, and unicellular zygotes. For this analysis, oocytes, eggs and embryos were spatially divided into peripheral and central regions of both the animal and vegetal hemispheres, and the relative amounts and concentrations of these RNAs in each region were then determined. The concentration of actin and tubulin mRNAs is greatest in the periphery, whereas histone mRNA exhibits a uniform concentration throughout the oocyte. In the meiotically mature egg, actin mRNA is still concentrated in the periphery and histone mRNA still exhibits a relatively uniform concentration, but the tubulin mRNAs are more concentrated in the central regions. Following fertilization, however, the greatest concentration of mRNAs for actin, histone and tubulin is in the periphery of the zygote. The results demonstrate the existence of a system capable of altering the distributions of these mRNAs as well as a system which distinguishes between different types of mRNA.",
keywords = "Cortex, Egg, mRNA, Xenopus",
author = "Perry, {Brian A.} and David Capco",
year = "1988",
doi = "10.1016/0922-3371(88)90003-2",
language = "English (US)",
volume = "25",
pages = "99--108",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Spatial reorganization of actin, tubulin and histone mRNAs during meiotic maturation and fertilization in Xenopus oocytes

AU - Perry, Brian A.

AU - Capco, David

PY - 1988

Y1 - 1988

N2 - The distribution of actin, tubulin and histone mRNAs is examined in full grown oocytes, meiotically mature eggs, and unicellular zygotes. For this analysis, oocytes, eggs and embryos were spatially divided into peripheral and central regions of both the animal and vegetal hemispheres, and the relative amounts and concentrations of these RNAs in each region were then determined. The concentration of actin and tubulin mRNAs is greatest in the periphery, whereas histone mRNA exhibits a uniform concentration throughout the oocyte. In the meiotically mature egg, actin mRNA is still concentrated in the periphery and histone mRNA still exhibits a relatively uniform concentration, but the tubulin mRNAs are more concentrated in the central regions. Following fertilization, however, the greatest concentration of mRNAs for actin, histone and tubulin is in the periphery of the zygote. The results demonstrate the existence of a system capable of altering the distributions of these mRNAs as well as a system which distinguishes between different types of mRNA.

AB - The distribution of actin, tubulin and histone mRNAs is examined in full grown oocytes, meiotically mature eggs, and unicellular zygotes. For this analysis, oocytes, eggs and embryos were spatially divided into peripheral and central regions of both the animal and vegetal hemispheres, and the relative amounts and concentrations of these RNAs in each region were then determined. The concentration of actin and tubulin mRNAs is greatest in the periphery, whereas histone mRNA exhibits a uniform concentration throughout the oocyte. In the meiotically mature egg, actin mRNA is still concentrated in the periphery and histone mRNA still exhibits a relatively uniform concentration, but the tubulin mRNAs are more concentrated in the central regions. Following fertilization, however, the greatest concentration of mRNAs for actin, histone and tubulin is in the periphery of the zygote. The results demonstrate the existence of a system capable of altering the distributions of these mRNAs as well as a system which distinguishes between different types of mRNA.

KW - Cortex

KW - Egg

KW - mRNA

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=0023775068&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023775068&partnerID=8YFLogxK

U2 - 10.1016/0922-3371(88)90003-2

DO - 10.1016/0922-3371(88)90003-2

M3 - Article

C2 - 3208194

AN - SCOPUS:0023775068

VL - 25

SP - 99

EP - 108

JO - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

IS - 2

ER -