Spaceflight modulates gene expression in the whole blood of astronauts

Jennifer Barrila, C. Mark Ott, Carly Leblanc, Satish K. Mehta, Aurélie Crabbé, Phillip Stafford, Duane L. Pierson, Cheryl Nickerson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Astronauts are exposed to a unique combination of stressors during spaceflight, which leads to alterations in their physiology and potentially increases their susceptibility to disease, including infectious diseases. To evaluate the potential impact of the spaceflight environment on the regulation of molecular pathways mediating cellular stress responses, we performed a first-of-its-kind pilot study to assess spaceflight-related gene-expression changes in the whole blood of astronauts. Using an array comprised of 234 well-characterized stress-response genes, we profiled transcriptomic changes in six astronauts (four men and two women) from blood preserved before and immediately following the spaceflight. Differentially regulated transcripts included those important for DNA repair, oxidative stress, and protein folding/degradation, including HSP90AB1, HSP27, GPX1, XRCC1, BAG-1, HHR23A, FAP48, and C-FOS. No gender-specific differences or relationship to number of missions flown was observed. This study provides a first assessment of transcriptomic changes occurring in the whole blood of astronauts in response to spaceflight.

Original languageEnglish (US)
Article number16039
Journalnpj Microgravity
Volume2
DOIs
StatePublished - Jan 7 2016

Fingerprint

Astronauts
space flight
Space Flight
astronauts
gene expression
Gene expression
blood
Blood
Gene Expression
Protein folding
transcriptomics
Oxidative stress
Physiology
infectious disease
Heat-Shock Proteins
stress response
repair
physiology
folding
gender

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)
  • Space and Planetary Science
  • Medicine (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Materials Science (miscellaneous)

Cite this

Spaceflight modulates gene expression in the whole blood of astronauts. / Barrila, Jennifer; Ott, C. Mark; Leblanc, Carly; Mehta, Satish K.; Crabbé, Aurélie; Stafford, Phillip; Pierson, Duane L.; Nickerson, Cheryl.

In: npj Microgravity, Vol. 2, 16039, 07.01.2016.

Research output: Contribution to journalArticle

Barrila, Jennifer ; Ott, C. Mark ; Leblanc, Carly ; Mehta, Satish K. ; Crabbé, Aurélie ; Stafford, Phillip ; Pierson, Duane L. ; Nickerson, Cheryl. / Spaceflight modulates gene expression in the whole blood of astronauts. In: npj Microgravity. 2016 ; Vol. 2.
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