Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq

J. W. Wilson; C. M. Ott; K. Höner Zu Bentrup; R. Ramamurthy; L. Quick; S. Porwollik; P. Cheng; M. McClelland; G. Tsaprailis; T. Radabaugh; A. Hunt; D. Fernandez; E. Richter; M. Shah; M. Kilcoyne; L. Joshi; M. Nelman-Gonzalez; S. Hing; M. Parra; P. Dumars; K. Norwood; R. Bober; J. Devich; A. Ruggles; C. Goulart; M. Rupert; L. Stodieck; Phillip Stafford; L. Catella; M. J. Schurr; K. Buchananb; L. Morici; J. McCracken; P. Allen; C. Baker-Coleman; T. Hammond; J. Vogel; R. Nelson; D. L. Pierson; H. M. Stefanyshyn-Piper; Cheryl Nickerson

doi:10.1073/pnas.0707155104

Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq

J. W. Wilson, C. M. Ott, K. Höner Zu Bentrup, R. Ramamurthy, L. Quick, S. Porwollik, P. Cheng, M. McClelland, G. Tsaprailis, T. Radabaugh, A. Hunt, D. Fernandez, E. Richter, M. Shah, M. Kilcoyne, L. Joshi, M. Nelman-Gonzalez, S. Hing, M. Parra, P. DumarsK. Norwood, R. Bober, J. Devich, A. Ruggles, C. Goulart, M. Rupert, L. Stodieck, Phillip Stafford, L. Catella, M. J. Schurr, K. Buchananb, L. Morici, J. McCracken, P. Allen, C. Baker-Coleman, T. Hammond, J. Vogel, R. Nelson, D. L. Pierson, H. M. Stefanyshyn-Piper, Cheryl Nickerson

Research output: Contribution to journal › Article › peer-review

395 Scopus citations

Abstract

A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and logistical hurdles. Moreover, the effects of space flight on microbial pathogenicity and associated infectious disease risks have not been studied. The bacterial pathogen Salmonella typhimurium was grown aboard Space Shuttle mission STS-115 and compared with identical ground control cultures. Global microarray and proteomic analyses revealed that 167 transcripts and 73 proteins changed expression with the conserved RNA-binding protein Hfq identified as a likely global regulator involved in the response to this environment. Hfq involvement was confirmed with a ground-based microgravity culture model. Space flight samples exhibited enhanced virulence in a murine infection model and extracellular matrix accumulation consistent with a biofilm. Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth.

Original language	English (US)
Pages (from-to)	16299-16304
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	104
Issue number	41
DOIs	https://doi.org/10.1073/pnas.0707155104
State	Published - Oct 9 2007

Keywords

Low shear modeled microgravity
Microgravity
Rotating wall vessel
Salmonella
Space shuttle

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.0707155104

Cite this

Wilson, J. W., Ott, C. M., Höner Zu Bentrup, K., Ramamurthy, R., Quick, L., Porwollik, S., Cheng, P., McClelland, M., Tsaprailis, G., Radabaugh, T., Hunt, A., Fernandez, D., Richter, E., Shah, M., Kilcoyne, M., Joshi, L., Nelman-Gonzalez, M., Hing, S., Parra, M., ... Nickerson, C. (2007). Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq. Proceedings of the National Academy of Sciences of the United States of America, 104(41), 16299-16304. https://doi.org/10.1073/pnas.0707155104

Wilson, JW, Ott, CM, Höner Zu Bentrup, K, Ramamurthy, R, Quick, L, Porwollik, S, Cheng, P, McClelland, M, Tsaprailis, G, Radabaugh, T, Hunt, A, Fernandez, D, Richter, E, Shah, M, Kilcoyne, M, Joshi, L, Nelman-Gonzalez, M, Hing, S, Parra, M, Dumars, P, Norwood, K, Bober, R, Devich, J, Ruggles, A, Goulart, C, Rupert, M, Stodieck, L, Stafford, P, Catella, L, Schurr, MJ, Buchananb, K, Morici, L, McCracken, J, Allen, P, Baker-Coleman, C, Hammond, T, Vogel, J, Nelson, R, Pierson, DL, Stefanyshyn-Piper, HM & Nickerson, C 2007, 'Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 41, pp. 16299-16304. https://doi.org/10.1073/pnas.0707155104

@article{22061129b67d476eaed82d23ee4c877c,

title = "Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq",

abstract = "A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and logistical hurdles. Moreover, the effects of space flight on microbial pathogenicity and associated infectious disease risks have not been studied. The bacterial pathogen Salmonella typhimurium was grown aboard Space Shuttle mission STS-115 and compared with identical ground control cultures. Global microarray and proteomic analyses revealed that 167 transcripts and 73 proteins changed expression with the conserved RNA-binding protein Hfq identified as a likely global regulator involved in the response to this environment. Hfq involvement was confirmed with a ground-based microgravity culture model. Space flight samples exhibited enhanced virulence in a murine infection model and extracellular matrix accumulation consistent with a biofilm. Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth.",

keywords = "Low shear modeled microgravity, Microgravity, Rotating wall vessel, Salmonella, Space shuttle",

author = "Wilson, {J. W.} and Ott, {C. M.} and {H{\"o}ner Zu Bentrup}, K. and R. Ramamurthy and L. Quick and S. Porwollik and P. Cheng and M. McClelland and G. Tsaprailis and T. Radabaugh and A. Hunt and D. Fernandez and E. Richter and M. Shah and M. Kilcoyne and L. Joshi and M. Nelman-Gonzalez and S. Hing and M. Parra and P. Dumars and K. Norwood and R. Bober and J. Devich and A. Ruggles and C. Goulart and M. Rupert and L. Stodieck and Phillip Stafford and L. Catella and Schurr, {M. J.} and K. Buchananb and L. Morici and J. McCracken and P. Allen and C. Baker-Coleman and T. Hammond and J. Vogel and R. Nelson and Pierson, {D. L.} and Stefanyshyn-Piper, {H. M.} and Cheryl Nickerson",

year = "2007",

month = oct,

day = "9",

doi = "10.1073/pnas.0707155104",

language = "English (US)",

volume = "104",

pages = "16299--16304",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "41",

}

TY - JOUR

T1 - Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq

AU - Wilson, J. W.

AU - Ott, C. M.

AU - Höner Zu Bentrup, K.

AU - Ramamurthy, R.

AU - Quick, L.

AU - Porwollik, S.

AU - Cheng, P.

AU - McClelland, M.

AU - Tsaprailis, G.

AU - Radabaugh, T.

AU - Hunt, A.

AU - Fernandez, D.

AU - Richter, E.

AU - Shah, M.

AU - Kilcoyne, M.

AU - Joshi, L.

AU - Nelman-Gonzalez, M.

AU - Hing, S.

AU - Parra, M.

AU - Dumars, P.

AU - Norwood, K.

AU - Bober, R.

AU - Devich, J.

AU - Ruggles, A.

AU - Goulart, C.

AU - Rupert, M.

AU - Stodieck, L.

AU - Stafford, Phillip

AU - Catella, L.

AU - Schurr, M. J.

AU - Buchananb, K.

AU - Morici, L.

AU - McCracken, J.

AU - Allen, P.

AU - Baker-Coleman, C.

AU - Hammond, T.

AU - Vogel, J.

AU - Nelson, R.

AU - Pierson, D. L.

AU - Stefanyshyn-Piper, H. M.

AU - Nickerson, Cheryl

PY - 2007/10/9

Y1 - 2007/10/9

N2 - A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and logistical hurdles. Moreover, the effects of space flight on microbial pathogenicity and associated infectious disease risks have not been studied. The bacterial pathogen Salmonella typhimurium was grown aboard Space Shuttle mission STS-115 and compared with identical ground control cultures. Global microarray and proteomic analyses revealed that 167 transcripts and 73 proteins changed expression with the conserved RNA-binding protein Hfq identified as a likely global regulator involved in the response to this environment. Hfq involvement was confirmed with a ground-based microgravity culture model. Space flight samples exhibited enhanced virulence in a murine infection model and extracellular matrix accumulation consistent with a biofilm. Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth.

AB - A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and logistical hurdles. Moreover, the effects of space flight on microbial pathogenicity and associated infectious disease risks have not been studied. The bacterial pathogen Salmonella typhimurium was grown aboard Space Shuttle mission STS-115 and compared with identical ground control cultures. Global microarray and proteomic analyses revealed that 167 transcripts and 73 proteins changed expression with the conserved RNA-binding protein Hfq identified as a likely global regulator involved in the response to this environment. Hfq involvement was confirmed with a ground-based microgravity culture model. Space flight samples exhibited enhanced virulence in a murine infection model and extracellular matrix accumulation consistent with a biofilm. Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth.

KW - Low shear modeled microgravity

KW - Microgravity

KW - Rotating wall vessel

KW - Salmonella

KW - Space shuttle

UR - http://www.scopus.com/inward/record.url?scp=36049010104&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36049010104&partnerID=8YFLogxK

U2 - 10.1073/pnas.0707155104

DO - 10.1073/pnas.0707155104

M3 - Article

C2 - 17901201

AN - SCOPUS:36049010104

SN - 0027-8424

VL - 104

SP - 16299

EP - 16304

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 41

ER -

Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this