Somitogenesis and axial development in reptiles

Cindy Xu, Mariana B. Grizante, Kenro Kusumi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Among amniote vertebrates, reptiles display the greatest variation in axial skeleton morphology. Only recently have they been used in gene expression studies of somitogenesis, challenging previous assumptions about the segmentation clock and axial patterning. An increasing number of reptile genomes and transcriptomes are becoming available as next-generation sequencing becomes more affordable. Information regarding gene sequence and structure can be used to design and synthesize labeled riboprobes by in vitro transcription for gene expression analysis by in situ hybridization, thus, enabling the characterization of spatial and temporal expression patterns of genes involved in somitogenesis, a topic of great interest within evolutionary developmental studies of vertebrates.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages335-353
Number of pages19
Volume1650
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1650
ISSN (Print)1064-3745

Keywords

  • Axial skeleton
  • Development
  • In situ hybridization
  • In vitro transcription
  • Reptile
  • Ribs
  • Somitogenesis
  • Vertebra

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Xu, C., Grizante, M. B., & Kusumi, K. (2017). Somitogenesis and axial development in reptiles. In Methods in Molecular Biology (Vol. 1650, pp. 335-353). (Methods in Molecular Biology; Vol. 1650). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7216-6_23