Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibres

Sarah G. Swygert, Benjamin J. Manning, Subhadip Senapati, Parminder Kaur, Stuart Lindsay, Borries Demeler, Craig L. Peterson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Heterochromatin is a repressive chromatin compartment essential for maintaining genomic integrity. A hallmark of heterochromatin is the presence of specialized nonhistone proteins that alter chromatin structure to inhibit transcription and recombination. It is generally assumed that heterochromatin is highly condensed. However, surprisingly little is known about the structure of heterochromatin or its dynamics in solution. In budding yeast, formation of heterochromatin at telomeres and the homothallic silent mating type loci require the Sir3 protein. Here, we use a combination of sedimentation velocity, atomic force microscopy and nucleosomal array capture to characterize the stoichiometry and conformation of Sir3 nucleosomal arrays. The results indicate that Sir3 interacts with nucleosomal arrays with a stoichiometry of two Sir3 monomers per nucleosome. We also find that Sir3 fibres are less compact than canonical magnesium-induced 30 nm fibres. We suggest that heterochromatin proteins promote silencing by 'coating' nucleosomal arrays, stabilizing interactions between nucleosomal histones and DNA.

Original languageEnglish (US)
Article number4751
JournalNature Communications
Volume5
DOIs
StatePublished - Aug 28 2014

Fingerprint

Heterochromatin
yeast
Stoichiometry
Yeast
Conformations
stoichiometry
Yeasts
chromatin
Chromatin
fibers
Fibers
proteins
Proteins
Nucleosomes
telomeres
Transcription
Sedimentation
Histones
Magnesium
Atomic force microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Swygert, S. G., Manning, B. J., Senapati, S., Kaur, P., Lindsay, S., Demeler, B., & Peterson, C. L. (2014). Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibres. Nature Communications, 5, [4751]. https://doi.org/10.1038/ncomms5751

Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibres. / Swygert, Sarah G.; Manning, Benjamin J.; Senapati, Subhadip; Kaur, Parminder; Lindsay, Stuart; Demeler, Borries; Peterson, Craig L.

In: Nature Communications, Vol. 5, 4751, 28.08.2014.

Research output: Contribution to journalArticle

Swygert, SG, Manning, BJ, Senapati, S, Kaur, P, Lindsay, S, Demeler, B & Peterson, CL 2014, 'Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibres', Nature Communications, vol. 5, 4751. https://doi.org/10.1038/ncomms5751
Swygert, Sarah G. ; Manning, Benjamin J. ; Senapati, Subhadip ; Kaur, Parminder ; Lindsay, Stuart ; Demeler, Borries ; Peterson, Craig L. / Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibres. In: Nature Communications. 2014 ; Vol. 5.
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