Soluble microbial products (SMP) in anaerobic chemostats

D. R. Noguera, N. Araki, Bruce Rittmann

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

The production of soluble microbial products (SMP) in anaerobic systems was evaluated using chemostat reactors. Results from steady-state and tracer experiments with 14C-glucose and 14C-acetate showed that significant amounts of SMP were produced during the acidogenesis of glucose, but that SMP did not accumulate during methanogenesis from acetate. In addition, at a retention time of 40 days, SMP comprised almost all of the effluent COD from the glucose-fed chemostat. For shorter retention times, as low as 10 days, the SMP concentration remained almost constant, but its significance in the effluent COD was reduced due to the accumulation of intermediate volatile fatty acids. The results from a 14C-tacer experiment in the glucose-fed chemostat were used to evaluate the importance of including SMP formation and degradation in kinetic modeling of the methanogenic chemostats. Three models were evaluated: a model without SMP production, a model with SMP production but no degradation, and a model with SMP production and degradation. The results of this kinetic analysis indicate that the model that includes SMP production and degradation was the only one able to adequately represent the fate of 14C in the tracer experiment. The kinetic parameters obtained from fitting the model to the tracer experiment were successfully used to predict steady-state concentrations of SMP and to characterize the formation and degradation characteristics of the SMP.

Original languageEnglish (US)
Pages (from-to)1040-1047
Number of pages8
JournalBiotechnology and Bioengineering
Volume44
Issue number9
DOIs
StatePublished - Nov 5 1994
Externally publishedYes

Fingerprint

Chemostats
Glucose
Degradation
Acetates
Volatile Fatty Acids
Effluents
Experiments
Volatile fatty acids
Kinetics
Kinetic parameters

Keywords

  • C-tracer experiments
  • Anaerobic chemostats
  • Biomass-associated products (BAP)
  • Kinetic modeling
  • Soluble microbial products (SMP)
  • Utilization-associated products (UAP)

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Soluble microbial products (SMP) in anaerobic chemostats. / Noguera, D. R.; Araki, N.; Rittmann, Bruce.

In: Biotechnology and Bioengineering, Vol. 44, No. 9, 05.11.1994, p. 1040-1047.

Research output: Contribution to journalArticle

Noguera, D. R. ; Araki, N. ; Rittmann, Bruce. / Soluble microbial products (SMP) in anaerobic chemostats. In: Biotechnology and Bioengineering. 1994 ; Vol. 44, No. 9. pp. 1040-1047.
@article{5fa943b091cd4d14876d71c7598ff895,
title = "Soluble microbial products (SMP) in anaerobic chemostats",
abstract = "The production of soluble microbial products (SMP) in anaerobic systems was evaluated using chemostat reactors. Results from steady-state and tracer experiments with 14C-glucose and 14C-acetate showed that significant amounts of SMP were produced during the acidogenesis of glucose, but that SMP did not accumulate during methanogenesis from acetate. In addition, at a retention time of 40 days, SMP comprised almost all of the effluent COD from the glucose-fed chemostat. For shorter retention times, as low as 10 days, the SMP concentration remained almost constant, but its significance in the effluent COD was reduced due to the accumulation of intermediate volatile fatty acids. The results from a 14C-tacer experiment in the glucose-fed chemostat were used to evaluate the importance of including SMP formation and degradation in kinetic modeling of the methanogenic chemostats. Three models were evaluated: a model without SMP production, a model with SMP production but no degradation, and a model with SMP production and degradation. The results of this kinetic analysis indicate that the model that includes SMP production and degradation was the only one able to adequately represent the fate of 14C in the tracer experiment. The kinetic parameters obtained from fitting the model to the tracer experiment were successfully used to predict steady-state concentrations of SMP and to characterize the formation and degradation characteristics of the SMP.",
keywords = "C-tracer experiments, Anaerobic chemostats, Biomass-associated products (BAP), Kinetic modeling, Soluble microbial products (SMP), Utilization-associated products (UAP)",
author = "Noguera, {D. R.} and N. Araki and Bruce Rittmann",
year = "1994",
month = "11",
day = "5",
doi = "10.1002/bit.260440904",
language = "English (US)",
volume = "44",
pages = "1040--1047",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "9",

}

TY - JOUR

T1 - Soluble microbial products (SMP) in anaerobic chemostats

AU - Noguera, D. R.

AU - Araki, N.

AU - Rittmann, Bruce

PY - 1994/11/5

Y1 - 1994/11/5

N2 - The production of soluble microbial products (SMP) in anaerobic systems was evaluated using chemostat reactors. Results from steady-state and tracer experiments with 14C-glucose and 14C-acetate showed that significant amounts of SMP were produced during the acidogenesis of glucose, but that SMP did not accumulate during methanogenesis from acetate. In addition, at a retention time of 40 days, SMP comprised almost all of the effluent COD from the glucose-fed chemostat. For shorter retention times, as low as 10 days, the SMP concentration remained almost constant, but its significance in the effluent COD was reduced due to the accumulation of intermediate volatile fatty acids. The results from a 14C-tacer experiment in the glucose-fed chemostat were used to evaluate the importance of including SMP formation and degradation in kinetic modeling of the methanogenic chemostats. Three models were evaluated: a model without SMP production, a model with SMP production but no degradation, and a model with SMP production and degradation. The results of this kinetic analysis indicate that the model that includes SMP production and degradation was the only one able to adequately represent the fate of 14C in the tracer experiment. The kinetic parameters obtained from fitting the model to the tracer experiment were successfully used to predict steady-state concentrations of SMP and to characterize the formation and degradation characteristics of the SMP.

AB - The production of soluble microbial products (SMP) in anaerobic systems was evaluated using chemostat reactors. Results from steady-state and tracer experiments with 14C-glucose and 14C-acetate showed that significant amounts of SMP were produced during the acidogenesis of glucose, but that SMP did not accumulate during methanogenesis from acetate. In addition, at a retention time of 40 days, SMP comprised almost all of the effluent COD from the glucose-fed chemostat. For shorter retention times, as low as 10 days, the SMP concentration remained almost constant, but its significance in the effluent COD was reduced due to the accumulation of intermediate volatile fatty acids. The results from a 14C-tacer experiment in the glucose-fed chemostat were used to evaluate the importance of including SMP formation and degradation in kinetic modeling of the methanogenic chemostats. Three models were evaluated: a model without SMP production, a model with SMP production but no degradation, and a model with SMP production and degradation. The results of this kinetic analysis indicate that the model that includes SMP production and degradation was the only one able to adequately represent the fate of 14C in the tracer experiment. The kinetic parameters obtained from fitting the model to the tracer experiment were successfully used to predict steady-state concentrations of SMP and to characterize the formation and degradation characteristics of the SMP.

KW - C-tracer experiments

KW - Anaerobic chemostats

KW - Biomass-associated products (BAP)

KW - Kinetic modeling

KW - Soluble microbial products (SMP)

KW - Utilization-associated products (UAP)

UR - http://www.scopus.com/inward/record.url?scp=0028765769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028765769&partnerID=8YFLogxK

U2 - 10.1002/bit.260440904

DO - 10.1002/bit.260440904

M3 - Article

VL - 44

SP - 1040

EP - 1047

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 9

ER -