Soluble corn fiber increases calcium absorption associated with shifts in the gut microbiome

A randomized dose-response trial in free-living pubertal females

Corrie Whisner, Berdine R. Martin, Cindy H. Nakatsu, Jon A. Story, Claire J. MacDonald-Clarke, Linda D. McCabe, George P. McCabe, Connie M. Weaver

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Soluble corn fiber (SCF; 12 g fiber/d) is shown to increase calcium absorption efficiency, associated with shifts in the gut microbiota in adolescent males and females who participated in a controlled feeding study. Objective: We evaluated the dose response of 0, 10, and 20 g fiber/d delivered by PROMITOR SCF 85 (85% fiber) on calcium absorption, biochemical bone properties, and the fecal microbiome in free-living adolescents. Methods: Healthy adolescent females (n = 28; aged 11-14 y) randomly assigned into a 3-phase, double-blind, crossover study consumed SCF for 4 wk at each dose (0, 10, and 20 g fiber/d from SCF) alongside their habitual diet and were followed by 3-d clinical visits and 3-wk washout periods. Stable isotope (44Ca and 43Ca) enrichment in pooled urine was measured by inductively coupled plasma mass spectrometry. Fecal microbial community composition was assessed by high-throughput sequencing (Illumina) of polymerase chain reaction-amplified 16S rRNA genes. Mixed model ANOVA and Friedman analysis were used to determine effects of SCF on calcium absorption and to compare mean microbial proportions, respectively. Results: Calciumabsorption increased significantlywith 10 (13.3%± 5.3%; P = 0.042) and 20 g fiber/d (12.9%± 3.6%; P = 0.026) from SCF relative to control. Significant differences in fecalmicrobial community diversitywere found after consuming SCF (operational taxonomic unit measures of 601.4 ± 83.5, 634.5 ± 83.8, and 649.6 ± 75.5 for 0, 10, and 20 g fiber/d, respectively; P <0.05). Proportions of the genus Parabacteroides significantly increased with SCF dose (1.1% ± 0.8%, 2.1%± 1.6%, and 3.0%± 2.0%for 0, 10, and 20 g fiber/d from SCF, respectively; P <0.05). Increases in calciumabsorption positively correlated with increases in Clostridium (r = 0.44, P = 0.023) and unclassified Clostridiaceae (r = 0.40, P = 0.040). Conclusions: SCF, a nondigestible carbohydrate, increased calcium absorption in free-living adolescent females. Two groups of bacteria may be involved, one directly fermenting SCF and the second fermenting SCF metabolites further, thereby promoting increased calcium absorption.

Original languageEnglish (US)
Pages (from-to)1298-1306
Number of pages9
JournalJournal of Nutrition
Volume146
Issue number7
DOIs
StatePublished - 2016

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Zea mays
Calcium
Clostridium
Microbiota
rRNA Genes
Double-Blind Method
Isotopes
Cross-Over Studies
Mass Spectrometry
Analysis of Variance
Carbohydrates
Gastrointestinal Microbiome
Urine
Diet
Bacteria
Bone and Bones
Polymerase Chain Reaction

Keywords

  • Adolescent
  • Bone health
  • Calcium
  • Microbiome
  • Osteoporosis
  • Prebiotic
  • Short-chain fatty acids

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Soluble corn fiber increases calcium absorption associated with shifts in the gut microbiome : A randomized dose-response trial in free-living pubertal females. / Whisner, Corrie; Martin, Berdine R.; Nakatsu, Cindy H.; Story, Jon A.; MacDonald-Clarke, Claire J.; McCabe, Linda D.; McCabe, George P.; Weaver, Connie M.

In: Journal of Nutrition, Vol. 146, No. 7, 2016, p. 1298-1306.

Research output: Contribution to journalArticle

Whisner, Corrie ; Martin, Berdine R. ; Nakatsu, Cindy H. ; Story, Jon A. ; MacDonald-Clarke, Claire J. ; McCabe, Linda D. ; McCabe, George P. ; Weaver, Connie M. / Soluble corn fiber increases calcium absorption associated with shifts in the gut microbiome : A randomized dose-response trial in free-living pubertal females. In: Journal of Nutrition. 2016 ; Vol. 146, No. 7. pp. 1298-1306.
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title = "Soluble corn fiber increases calcium absorption associated with shifts in the gut microbiome: A randomized dose-response trial in free-living pubertal females",
abstract = "Background: Soluble corn fiber (SCF; 12 g fiber/d) is shown to increase calcium absorption efficiency, associated with shifts in the gut microbiota in adolescent males and females who participated in a controlled feeding study. Objective: We evaluated the dose response of 0, 10, and 20 g fiber/d delivered by PROMITOR SCF 85 (85{\%} fiber) on calcium absorption, biochemical bone properties, and the fecal microbiome in free-living adolescents. Methods: Healthy adolescent females (n = 28; aged 11-14 y) randomly assigned into a 3-phase, double-blind, crossover study consumed SCF for 4 wk at each dose (0, 10, and 20 g fiber/d from SCF) alongside their habitual diet and were followed by 3-d clinical visits and 3-wk washout periods. Stable isotope (44Ca and 43Ca) enrichment in pooled urine was measured by inductively coupled plasma mass spectrometry. Fecal microbial community composition was assessed by high-throughput sequencing (Illumina) of polymerase chain reaction-amplified 16S rRNA genes. Mixed model ANOVA and Friedman analysis were used to determine effects of SCF on calcium absorption and to compare mean microbial proportions, respectively. Results: Calciumabsorption increased significantlywith 10 (13.3{\%}± 5.3{\%}; P = 0.042) and 20 g fiber/d (12.9{\%}± 3.6{\%}; P = 0.026) from SCF relative to control. Significant differences in fecalmicrobial community diversitywere found after consuming SCF (operational taxonomic unit measures of 601.4 ± 83.5, 634.5 ± 83.8, and 649.6 ± 75.5 for 0, 10, and 20 g fiber/d, respectively; P <0.05). Proportions of the genus Parabacteroides significantly increased with SCF dose (1.1{\%} ± 0.8{\%}, 2.1{\%}± 1.6{\%}, and 3.0{\%}± 2.0{\%}for 0, 10, and 20 g fiber/d from SCF, respectively; P <0.05). Increases in calciumabsorption positively correlated with increases in Clostridium (r = 0.44, P = 0.023) and unclassified Clostridiaceae (r = 0.40, P = 0.040). Conclusions: SCF, a nondigestible carbohydrate, increased calcium absorption in free-living adolescent females. Two groups of bacteria may be involved, one directly fermenting SCF and the second fermenting SCF metabolites further, thereby promoting increased calcium absorption.",
keywords = "Adolescent, Bone health, Calcium, Microbiome, Osteoporosis, Prebiotic, Short-chain fatty acids",
author = "Corrie Whisner and Martin, {Berdine R.} and Nakatsu, {Cindy H.} and Story, {Jon A.} and MacDonald-Clarke, {Claire J.} and McCabe, {Linda D.} and McCabe, {George P.} and Weaver, {Connie M.}",
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T1 - Soluble corn fiber increases calcium absorption associated with shifts in the gut microbiome

T2 - A randomized dose-response trial in free-living pubertal females

AU - Whisner, Corrie

AU - Martin, Berdine R.

AU - Nakatsu, Cindy H.

AU - Story, Jon A.

AU - MacDonald-Clarke, Claire J.

AU - McCabe, Linda D.

AU - McCabe, George P.

AU - Weaver, Connie M.

PY - 2016

Y1 - 2016

N2 - Background: Soluble corn fiber (SCF; 12 g fiber/d) is shown to increase calcium absorption efficiency, associated with shifts in the gut microbiota in adolescent males and females who participated in a controlled feeding study. Objective: We evaluated the dose response of 0, 10, and 20 g fiber/d delivered by PROMITOR SCF 85 (85% fiber) on calcium absorption, biochemical bone properties, and the fecal microbiome in free-living adolescents. Methods: Healthy adolescent females (n = 28; aged 11-14 y) randomly assigned into a 3-phase, double-blind, crossover study consumed SCF for 4 wk at each dose (0, 10, and 20 g fiber/d from SCF) alongside their habitual diet and were followed by 3-d clinical visits and 3-wk washout periods. Stable isotope (44Ca and 43Ca) enrichment in pooled urine was measured by inductively coupled plasma mass spectrometry. Fecal microbial community composition was assessed by high-throughput sequencing (Illumina) of polymerase chain reaction-amplified 16S rRNA genes. Mixed model ANOVA and Friedman analysis were used to determine effects of SCF on calcium absorption and to compare mean microbial proportions, respectively. Results: Calciumabsorption increased significantlywith 10 (13.3%± 5.3%; P = 0.042) and 20 g fiber/d (12.9%± 3.6%; P = 0.026) from SCF relative to control. Significant differences in fecalmicrobial community diversitywere found after consuming SCF (operational taxonomic unit measures of 601.4 ± 83.5, 634.5 ± 83.8, and 649.6 ± 75.5 for 0, 10, and 20 g fiber/d, respectively; P <0.05). Proportions of the genus Parabacteroides significantly increased with SCF dose (1.1% ± 0.8%, 2.1%± 1.6%, and 3.0%± 2.0%for 0, 10, and 20 g fiber/d from SCF, respectively; P <0.05). Increases in calciumabsorption positively correlated with increases in Clostridium (r = 0.44, P = 0.023) and unclassified Clostridiaceae (r = 0.40, P = 0.040). Conclusions: SCF, a nondigestible carbohydrate, increased calcium absorption in free-living adolescent females. Two groups of bacteria may be involved, one directly fermenting SCF and the second fermenting SCF metabolites further, thereby promoting increased calcium absorption.

AB - Background: Soluble corn fiber (SCF; 12 g fiber/d) is shown to increase calcium absorption efficiency, associated with shifts in the gut microbiota in adolescent males and females who participated in a controlled feeding study. Objective: We evaluated the dose response of 0, 10, and 20 g fiber/d delivered by PROMITOR SCF 85 (85% fiber) on calcium absorption, biochemical bone properties, and the fecal microbiome in free-living adolescents. Methods: Healthy adolescent females (n = 28; aged 11-14 y) randomly assigned into a 3-phase, double-blind, crossover study consumed SCF for 4 wk at each dose (0, 10, and 20 g fiber/d from SCF) alongside their habitual diet and were followed by 3-d clinical visits and 3-wk washout periods. Stable isotope (44Ca and 43Ca) enrichment in pooled urine was measured by inductively coupled plasma mass spectrometry. Fecal microbial community composition was assessed by high-throughput sequencing (Illumina) of polymerase chain reaction-amplified 16S rRNA genes. Mixed model ANOVA and Friedman analysis were used to determine effects of SCF on calcium absorption and to compare mean microbial proportions, respectively. Results: Calciumabsorption increased significantlywith 10 (13.3%± 5.3%; P = 0.042) and 20 g fiber/d (12.9%± 3.6%; P = 0.026) from SCF relative to control. Significant differences in fecalmicrobial community diversitywere found after consuming SCF (operational taxonomic unit measures of 601.4 ± 83.5, 634.5 ± 83.8, and 649.6 ± 75.5 for 0, 10, and 20 g fiber/d, respectively; P <0.05). Proportions of the genus Parabacteroides significantly increased with SCF dose (1.1% ± 0.8%, 2.1%± 1.6%, and 3.0%± 2.0%for 0, 10, and 20 g fiber/d from SCF, respectively; P <0.05). Increases in calciumabsorption positively correlated with increases in Clostridium (r = 0.44, P = 0.023) and unclassified Clostridiaceae (r = 0.40, P = 0.040). Conclusions: SCF, a nondigestible carbohydrate, increased calcium absorption in free-living adolescent females. Two groups of bacteria may be involved, one directly fermenting SCF and the second fermenting SCF metabolites further, thereby promoting increased calcium absorption.

KW - Adolescent

KW - Bone health

KW - Calcium

KW - Microbiome

KW - Osteoporosis

KW - Prebiotic

KW - Short-chain fatty acids

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