TY - JOUR
T1 - Soluble corn fiber increases calcium absorption associated with shifts in the gut microbiome
T2 - A randomized dose-response trial in free-living pubertal females
AU - Whisner, Corrie
AU - Martin, Berdine R.
AU - Nakatsu, Cindy H.
AU - Story, Jon A.
AU - MacDonald-Clarke, Claire J.
AU - McCabe, Linda D.
AU - McCabe, George P.
AU - Weaver, Connie M.
N1 - Publisher Copyright:
© 2016 American Society for Nutrition.
PY - 2016
Y1 - 2016
N2 - Background: Soluble corn fiber (SCF; 12 g fiber/d) is shown to increase calcium absorption efficiency, associated with shifts in the gut microbiota in adolescent males and females who participated in a controlled feeding study. Objective: We evaluated the dose response of 0, 10, and 20 g fiber/d delivered by PROMITOR SCF 85 (85% fiber) on calcium absorption, biochemical bone properties, and the fecal microbiome in free-living adolescents. Methods: Healthy adolescent females (n = 28; aged 11-14 y) randomly assigned into a 3-phase, double-blind, crossover study consumed SCF for 4 wk at each dose (0, 10, and 20 g fiber/d from SCF) alongside their habitual diet and were followed by 3-d clinical visits and 3-wk washout periods. Stable isotope (44Ca and 43Ca) enrichment in pooled urine was measured by inductively coupled plasma mass spectrometry. Fecal microbial community composition was assessed by high-throughput sequencing (Illumina) of polymerase chain reaction-amplified 16S rRNA genes. Mixed model ANOVA and Friedman analysis were used to determine effects of SCF on calcium absorption and to compare mean microbial proportions, respectively. Results: Calciumabsorption increased significantlywith 10 (13.3%± 5.3%; P = 0.042) and 20 g fiber/d (12.9%± 3.6%; P = 0.026) from SCF relative to control. Significant differences in fecalmicrobial community diversitywere found after consuming SCF (operational taxonomic unit measures of 601.4 ± 83.5, 634.5 ± 83.8, and 649.6 ± 75.5 for 0, 10, and 20 g fiber/d, respectively; P < 0.05). Proportions of the genus Parabacteroides significantly increased with SCF dose (1.1% ± 0.8%, 2.1%± 1.6%, and 3.0%± 2.0%for 0, 10, and 20 g fiber/d from SCF, respectively; P < 0.05). Increases in calciumabsorption positively correlated with increases in Clostridium (r = 0.44, P = 0.023) and unclassified Clostridiaceae (r = 0.40, P = 0.040). Conclusions: SCF, a nondigestible carbohydrate, increased calcium absorption in free-living adolescent females. Two groups of bacteria may be involved, one directly fermenting SCF and the second fermenting SCF metabolites further, thereby promoting increased calcium absorption.
AB - Background: Soluble corn fiber (SCF; 12 g fiber/d) is shown to increase calcium absorption efficiency, associated with shifts in the gut microbiota in adolescent males and females who participated in a controlled feeding study. Objective: We evaluated the dose response of 0, 10, and 20 g fiber/d delivered by PROMITOR SCF 85 (85% fiber) on calcium absorption, biochemical bone properties, and the fecal microbiome in free-living adolescents. Methods: Healthy adolescent females (n = 28; aged 11-14 y) randomly assigned into a 3-phase, double-blind, crossover study consumed SCF for 4 wk at each dose (0, 10, and 20 g fiber/d from SCF) alongside their habitual diet and were followed by 3-d clinical visits and 3-wk washout periods. Stable isotope (44Ca and 43Ca) enrichment in pooled urine was measured by inductively coupled plasma mass spectrometry. Fecal microbial community composition was assessed by high-throughput sequencing (Illumina) of polymerase chain reaction-amplified 16S rRNA genes. Mixed model ANOVA and Friedman analysis were used to determine effects of SCF on calcium absorption and to compare mean microbial proportions, respectively. Results: Calciumabsorption increased significantlywith 10 (13.3%± 5.3%; P = 0.042) and 20 g fiber/d (12.9%± 3.6%; P = 0.026) from SCF relative to control. Significant differences in fecalmicrobial community diversitywere found after consuming SCF (operational taxonomic unit measures of 601.4 ± 83.5, 634.5 ± 83.8, and 649.6 ± 75.5 for 0, 10, and 20 g fiber/d, respectively; P < 0.05). Proportions of the genus Parabacteroides significantly increased with SCF dose (1.1% ± 0.8%, 2.1%± 1.6%, and 3.0%± 2.0%for 0, 10, and 20 g fiber/d from SCF, respectively; P < 0.05). Increases in calciumabsorption positively correlated with increases in Clostridium (r = 0.44, P = 0.023) and unclassified Clostridiaceae (r = 0.40, P = 0.040). Conclusions: SCF, a nondigestible carbohydrate, increased calcium absorption in free-living adolescent females. Two groups of bacteria may be involved, one directly fermenting SCF and the second fermenting SCF metabolites further, thereby promoting increased calcium absorption.
KW - Adolescent
KW - Bone health
KW - Calcium
KW - Microbiome
KW - Osteoporosis
KW - Prebiotic
KW - Short-chain fatty acids
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UR - http://www.scopus.com/inward/citedby.url?scp=84977575173&partnerID=8YFLogxK
U2 - 10.3945/jn.115.227256
DO - 10.3945/jn.115.227256
M3 - Article
C2 - 27281813
AN - SCOPUS:84977575173
SN - 0022-3166
VL - 146
SP - 1298
EP - 1306
JO - Journal of Nutrition
JF - Journal of Nutrition
IS - 7
ER -