Solid-state NMR comparison of various spiders' dragline silk fiber

Melinda S. Creager, Janelle E. Jenkins, Leigh A. Thagard-Yeaman, Amanda E. Brooks, Justin A. Jones, Randolph V. Lewis, Gregory P. Holland, Jeffery Yarger

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Major ampullate (dragline) spider silk is a coveted biopolymer due to its combination of strength and extensibility. The dragline silk of different spiders have distinct mechanical properties that can be qualitatively correlated to the protein sequence. This study uses amino acid analysis and carbon-13 solid-state NMR to compare the molecular composition, structure, and dynamics of major ampullate dragline silk of four orb-web spider species (Nephila clavipes, Araneus gemmoides, Argiope aurantia, and Argiope argentata) and one cobweb species (Latrodectus hesperus). The mobility of the protein backbone and amino acid side chains in water exposed silk fibers is shown to correlate to the proline content. This implies that regions of major ampullate spidroin 2 protein, which is the only dragline silk protein with any significant proline content, become significantly hydrated in dragline spider silk.

Original languageEnglish (US)
Pages (from-to)2039-2043
Number of pages5
JournalBiomacromolecules
Volume11
Issue number8
DOIs
StatePublished - Aug 9 2010

Fingerprint

Silk
Nuclear magnetic resonance
Fibers
Proteins
Proline
Amino acids
Amino Acids
Biopolymers
Carbon
Mechanical properties
Water
Chemical analysis

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Creager, M. S., Jenkins, J. E., Thagard-Yeaman, L. A., Brooks, A. E., Jones, J. A., Lewis, R. V., ... Yarger, J. (2010). Solid-state NMR comparison of various spiders' dragline silk fiber. Biomacromolecules, 11(8), 2039-2043. https://doi.org/10.1021/bm100399x

Solid-state NMR comparison of various spiders' dragline silk fiber. / Creager, Melinda S.; Jenkins, Janelle E.; Thagard-Yeaman, Leigh A.; Brooks, Amanda E.; Jones, Justin A.; Lewis, Randolph V.; Holland, Gregory P.; Yarger, Jeffery.

In: Biomacromolecules, Vol. 11, No. 8, 09.08.2010, p. 2039-2043.

Research output: Contribution to journalArticle

Creager, MS, Jenkins, JE, Thagard-Yeaman, LA, Brooks, AE, Jones, JA, Lewis, RV, Holland, GP & Yarger, J 2010, 'Solid-state NMR comparison of various spiders' dragline silk fiber', Biomacromolecules, vol. 11, no. 8, pp. 2039-2043. https://doi.org/10.1021/bm100399x
Creager MS, Jenkins JE, Thagard-Yeaman LA, Brooks AE, Jones JA, Lewis RV et al. Solid-state NMR comparison of various spiders' dragline silk fiber. Biomacromolecules. 2010 Aug 9;11(8):2039-2043. https://doi.org/10.1021/bm100399x
Creager, Melinda S. ; Jenkins, Janelle E. ; Thagard-Yeaman, Leigh A. ; Brooks, Amanda E. ; Jones, Justin A. ; Lewis, Randolph V. ; Holland, Gregory P. ; Yarger, Jeffery. / Solid-state NMR comparison of various spiders' dragline silk fiber. In: Biomacromolecules. 2010 ; Vol. 11, No. 8. pp. 2039-2043.
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