Small-Angle Neutron Scattering of RNA–Protein Complexes

Audrone Lapinaite, Teresa Carlomagno, Frank Gabel

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Small-angle neutron scattering (SANS) provides structural information on biomacromolecules and their complexes in dilute solutions at the nanometer length scale. The overall dimensions, shapes, and interactions can be probed and compared to information obtained by complementary structural biology techniques such as crystallography, NMR, and EM. SANS, in combination with solvent H2O/D2O exchange and/or deuteration, is particularly well suited to probe the internal structure of RNA–protein (RNP) complexes since neutrons are more sensitive than X-rays to the difference in scattering length densities of proteins and RNA, with respect to an aqueous solvent. In this book chapter we provide a practical guide on how to carry out SANS experiments on RNP complexes, as well as possibilities of data analysis and interpretation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages165-188
Number of pages24
DOIs
StatePublished - 2020
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2113
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Contrast variation
  • Deuterium labeling
  • Macromolecular complex
  • Protein
  • RNA
  • RNP
  • Small-angle neutron scattering (SANS)
  • Structural biology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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