Slowing DNA translocation through a nanopore using a functionalized electrode

Padmini Krishnakumar, Brett Gyarfas, Weisi Song, Suman Sen, Peiming Zhang, Predrag Krstić, Stuart Lindsay

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Nanopores were fabricated with an integrated microscale Pd electrode coated with either a hydrogen-bonding or hydrophobic monolayer. Bare pores, or those coated with octanethiol, translocated single-stranded DNA with times of a few microseconds per base. Pores functionalized with 4(5)-(2-mercaptoethyl)-1H- imidazole-2-carboxamide slowed average translocation times, calculated as the duration of the event divided by the number of bases translocated, to about 100 μs per base at biases in the range of 50 to 80 mV.

Original languageEnglish (US)
Pages (from-to)10319-10326
Number of pages8
JournalACS Nano
Volume7
Issue number11
DOIs
StatePublished - Nov 26 2013

Fingerprint

Nanopores
Single-Stranded DNA
Monolayers
Hydrogen bonds
DNA
deoxyribonucleic acid
Electrodes
electrodes
porosity
imidazoles
microbalances
hydrogen
4(5)-(2-mercaptoethyl)-1H-imidazole-2-carboxamide

Keywords

  • DNA sequencing
  • DNA translocation
  • nanopore
  • recognition tunneling
  • slowing translocation

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Krishnakumar, P., Gyarfas, B., Song, W., Sen, S., Zhang, P., Krstić, P., & Lindsay, S. (2013). Slowing DNA translocation through a nanopore using a functionalized electrode. ACS Nano, 7(11), 10319-10326. https://doi.org/10.1021/nn404743f

Slowing DNA translocation through a nanopore using a functionalized electrode. / Krishnakumar, Padmini; Gyarfas, Brett; Song, Weisi; Sen, Suman; Zhang, Peiming; Krstić, Predrag; Lindsay, Stuart.

In: ACS Nano, Vol. 7, No. 11, 26.11.2013, p. 10319-10326.

Research output: Contribution to journalArticle

Krishnakumar, P, Gyarfas, B, Song, W, Sen, S, Zhang, P, Krstić, P & Lindsay, S 2013, 'Slowing DNA translocation through a nanopore using a functionalized electrode', ACS Nano, vol. 7, no. 11, pp. 10319-10326. https://doi.org/10.1021/nn404743f
Krishnakumar P, Gyarfas B, Song W, Sen S, Zhang P, Krstić P et al. Slowing DNA translocation through a nanopore using a functionalized electrode. ACS Nano. 2013 Nov 26;7(11):10319-10326. https://doi.org/10.1021/nn404743f
Krishnakumar, Padmini ; Gyarfas, Brett ; Song, Weisi ; Sen, Suman ; Zhang, Peiming ; Krstić, Predrag ; Lindsay, Stuart. / Slowing DNA translocation through a nanopore using a functionalized electrode. In: ACS Nano. 2013 ; Vol. 7, No. 11. pp. 10319-10326.
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