Slowing DNA translocation through a nanopore using a functionalized electrode

Padmini Krishnakumar; Brett Gyarfas; Weisi Song; Suman Sen; Peiming Zhang; Predrag Krstić; Stuart Lindsay

doi:10.1021/nn404743f

Slowing DNA translocation through a nanopore using a functionalized electrode

Padmini Krishnakumar, Brett Gyarfas, Weisi Song, Suman Sen, Peiming Zhang, Predrag Krstić, Stuart Lindsay

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Nanopores were fabricated with an integrated microscale Pd electrode coated with either a hydrogen-bonding or hydrophobic monolayer. Bare pores, or those coated with octanethiol, translocated single-stranded DNA with times of a few microseconds per base. Pores functionalized with 4(5)-(2-mercaptoethyl)-1H- imidazole-2-carboxamide slowed average translocation times, calculated as the duration of the event divided by the number of bases translocated, to about 100 μs per base at biases in the range of 50 to 80 mV.

Original language	English (US)
Pages (from-to)	10319-10326
Number of pages	8
Journal	ACS nano
Volume	7
Issue number	11
DOIs	https://doi.org/10.1021/nn404743f
State	Published - Nov 26 2013

Keywords

DNA sequencing
DNA translocation
nanopore
recognition tunneling
slowing translocation

ASJC Scopus subject areas

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)

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10.1021/nn404743f

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AU - Krishnakumar, Padmini

AU - Gyarfas, Brett

AU - Song, Weisi

AU - Sen, Suman

AU - Zhang, Peiming

AU - Krstić, Predrag

AU - Lindsay, Stuart

PY - 2013/11/26

Y1 - 2013/11/26

N2 - Nanopores were fabricated with an integrated microscale Pd electrode coated with either a hydrogen-bonding or hydrophobic monolayer. Bare pores, or those coated with octanethiol, translocated single-stranded DNA with times of a few microseconds per base. Pores functionalized with 4(5)-(2-mercaptoethyl)-1H- imidazole-2-carboxamide slowed average translocation times, calculated as the duration of the event divided by the number of bases translocated, to about 100 μs per base at biases in the range of 50 to 80 mV.

AB - Nanopores were fabricated with an integrated microscale Pd electrode coated with either a hydrogen-bonding or hydrophobic monolayer. Bare pores, or those coated with octanethiol, translocated single-stranded DNA with times of a few microseconds per base. Pores functionalized with 4(5)-(2-mercaptoethyl)-1H- imidazole-2-carboxamide slowed average translocation times, calculated as the duration of the event divided by the number of bases translocated, to about 100 μs per base at biases in the range of 50 to 80 mV.

KW - DNA sequencing

KW - DNA translocation

KW - nanopore

KW - recognition tunneling

KW - slowing translocation

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Slowing DNA translocation through a nanopore using a functionalized electrode

Abstract

Keywords

ASJC Scopus subject areas

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