Size exclusion chromatography with online ICP-MS enables molecular weight fractionation of dissolved phosphorus species in water samples

Arjun K. Venkatesan, Wenhui Gan, Harsh Ashani, Pierre Herckes, Paul Westerhoff

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Phosphorus (P) is an important and often limiting element in terrestrial and aquatic ecosystem. A lack of understanding of its distribution and structures in the environment limits the design of effective P mitigation and recovery approaches. Here we developed a robust method employing size exclusion chromatography (SEC) coupled to an ICP-MS to determine the molecular weight (MW) distribution of P in environmental samples. The most abundant fraction of P varied widely in different environmental samples: (i) orthophosphate was the dominant fraction (93–100%) in one lake, two aerosols and DOC isolate samples, (ii) species of 400–600 Da range were abundant (74–100%) in two surface waters, and (iii) species of 150–350 Da range were abundant in wastewater effluents. SEC-DOC of the aqueous samples using a similar SEC column showed overlapping peaks for the 400–600 Da species in two surface waters, and for >20 kDa species in the effluents, suggesting that these fractions are likely associated with organic matter. The MW resolution and performance of SEC-ICP-MS agreed well with the time integrated results obtained using conventional ultrafiltration method. Results show that SEC in combination with ICP-MS and DOC has the potential to be a powerful and easy-to-use method in identifying unknown fractions of P in the environment.

Original languageEnglish (US)
Pages (from-to)264-271
Number of pages8
JournalWater Research
Volume133
DOIs
StatePublished - Apr 15 2018

Fingerprint

Size exclusion chromatography
Fractionation
chromatography
Phosphorus
fractionation
Molecular weight
phosphorus
Water
Surface waters
Effluents
water
effluent
surface water
Aquatic ecosystems
orthophosphate
Ultrafiltration
Molecular weight distribution
ultrafiltration
terrestrial ecosystem
aquatic ecosystem

Keywords

  • Dissolved organic carbon
  • ICP-MS
  • Molecular weight distribution
  • Phosphorus
  • Size exclusion chromatography

ASJC Scopus subject areas

  • Ecological Modeling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

Size exclusion chromatography with online ICP-MS enables molecular weight fractionation of dissolved phosphorus species in water samples. / Venkatesan, Arjun K.; Gan, Wenhui; Ashani, Harsh; Herckes, Pierre; Westerhoff, Paul.

In: Water Research, Vol. 133, 15.04.2018, p. 264-271.

Research output: Contribution to journalArticle

@article{d27dfc2618bc405db930ec5424548ac2,
title = "Size exclusion chromatography with online ICP-MS enables molecular weight fractionation of dissolved phosphorus species in water samples",
abstract = "Phosphorus (P) is an important and often limiting element in terrestrial and aquatic ecosystem. A lack of understanding of its distribution and structures in the environment limits the design of effective P mitigation and recovery approaches. Here we developed a robust method employing size exclusion chromatography (SEC) coupled to an ICP-MS to determine the molecular weight (MW) distribution of P in environmental samples. The most abundant fraction of P varied widely in different environmental samples: (i) orthophosphate was the dominant fraction (93–100{\%}) in one lake, two aerosols and DOC isolate samples, (ii) species of 400–600 Da range were abundant (74–100{\%}) in two surface waters, and (iii) species of 150–350 Da range were abundant in wastewater effluents. SEC-DOC of the aqueous samples using a similar SEC column showed overlapping peaks for the 400–600 Da species in two surface waters, and for >20 kDa species in the effluents, suggesting that these fractions are likely associated with organic matter. The MW resolution and performance of SEC-ICP-MS agreed well with the time integrated results obtained using conventional ultrafiltration method. Results show that SEC in combination with ICP-MS and DOC has the potential to be a powerful and easy-to-use method in identifying unknown fractions of P in the environment.",
keywords = "Dissolved organic carbon, ICP-MS, Molecular weight distribution, Phosphorus, Size exclusion chromatography",
author = "Venkatesan, {Arjun K.} and Wenhui Gan and Harsh Ashani and Pierre Herckes and Paul Westerhoff",
year = "2018",
month = "4",
day = "15",
doi = "10.1016/j.watres.2018.01.048",
language = "English (US)",
volume = "133",
pages = "264--271",
journal = "Water Research",
issn = "0043-1354",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Size exclusion chromatography with online ICP-MS enables molecular weight fractionation of dissolved phosphorus species in water samples

AU - Venkatesan, Arjun K.

AU - Gan, Wenhui

AU - Ashani, Harsh

AU - Herckes, Pierre

AU - Westerhoff, Paul

PY - 2018/4/15

Y1 - 2018/4/15

N2 - Phosphorus (P) is an important and often limiting element in terrestrial and aquatic ecosystem. A lack of understanding of its distribution and structures in the environment limits the design of effective P mitigation and recovery approaches. Here we developed a robust method employing size exclusion chromatography (SEC) coupled to an ICP-MS to determine the molecular weight (MW) distribution of P in environmental samples. The most abundant fraction of P varied widely in different environmental samples: (i) orthophosphate was the dominant fraction (93–100%) in one lake, two aerosols and DOC isolate samples, (ii) species of 400–600 Da range were abundant (74–100%) in two surface waters, and (iii) species of 150–350 Da range were abundant in wastewater effluents. SEC-DOC of the aqueous samples using a similar SEC column showed overlapping peaks for the 400–600 Da species in two surface waters, and for >20 kDa species in the effluents, suggesting that these fractions are likely associated with organic matter. The MW resolution and performance of SEC-ICP-MS agreed well with the time integrated results obtained using conventional ultrafiltration method. Results show that SEC in combination with ICP-MS and DOC has the potential to be a powerful and easy-to-use method in identifying unknown fractions of P in the environment.

AB - Phosphorus (P) is an important and often limiting element in terrestrial and aquatic ecosystem. A lack of understanding of its distribution and structures in the environment limits the design of effective P mitigation and recovery approaches. Here we developed a robust method employing size exclusion chromatography (SEC) coupled to an ICP-MS to determine the molecular weight (MW) distribution of P in environmental samples. The most abundant fraction of P varied widely in different environmental samples: (i) orthophosphate was the dominant fraction (93–100%) in one lake, two aerosols and DOC isolate samples, (ii) species of 400–600 Da range were abundant (74–100%) in two surface waters, and (iii) species of 150–350 Da range were abundant in wastewater effluents. SEC-DOC of the aqueous samples using a similar SEC column showed overlapping peaks for the 400–600 Da species in two surface waters, and for >20 kDa species in the effluents, suggesting that these fractions are likely associated with organic matter. The MW resolution and performance of SEC-ICP-MS agreed well with the time integrated results obtained using conventional ultrafiltration method. Results show that SEC in combination with ICP-MS and DOC has the potential to be a powerful and easy-to-use method in identifying unknown fractions of P in the environment.

KW - Dissolved organic carbon

KW - ICP-MS

KW - Molecular weight distribution

KW - Phosphorus

KW - Size exclusion chromatography

UR - http://www.scopus.com/inward/record.url?scp=85041422949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041422949&partnerID=8YFLogxK

U2 - 10.1016/j.watres.2018.01.048

DO - 10.1016/j.watres.2018.01.048

M3 - Article

C2 - 29407707

AN - SCOPUS:85041422949

VL - 133

SP - 264

EP - 271

JO - Water Research

JF - Water Research

SN - 0043-1354

ER -