Size-dependent free solution DNA electrophoresis in structured microfluidic systems

Thanh Tu Duong, Gyuman Kim, Robert Ros, Martin Streek, Friederike Schmid, J. ürgen Brugger, Dario Anselmetti, Alexandra Ros

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (<3 μm) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that λ-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress.

Original languageEnglish (US)
Pages (from-to)905-912
Number of pages8
JournalMicroelectronic Engineering
Volume67-68
DOIs
StatePublished - Jun 2003
Externally publishedYes

Fingerprint

electrophoresis
Electrophoresis
Microfluidics
DNA
deoxyribonucleic acid
Molecules
Intercalating Agents
molecules
gyration
Polydimethylsiloxane
Photoresists
microchannels
Microchannels
photoresists
Molding
layouts
Lithography
Microscopic examination
lithography
Fluorescence

Keywords

  • Contact lithography
  • DNA
  • Electrophoresis
  • Fluorescence microscopy
  • Microchip
  • Single molecule

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Atomic and Molecular Physics, and Optics

Cite this

Size-dependent free solution DNA electrophoresis in structured microfluidic systems. / Duong, Thanh Tu; Kim, Gyuman; Ros, Robert; Streek, Martin; Schmid, Friederike; Brugger, J. ürgen; Anselmetti, Dario; Ros, Alexandra.

In: Microelectronic Engineering, Vol. 67-68, 06.2003, p. 905-912.

Research output: Contribution to journalArticle

Duong, Thanh Tu ; Kim, Gyuman ; Ros, Robert ; Streek, Martin ; Schmid, Friederike ; Brugger, J. ürgen ; Anselmetti, Dario ; Ros, Alexandra. / Size-dependent free solution DNA electrophoresis in structured microfluidic systems. In: Microelectronic Engineering. 2003 ; Vol. 67-68. pp. 905-912.
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