Abstract
A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (<3 μm) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that λ-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 905-912 |
| Number of pages | 8 |
| Journal | Microelectronic Engineering |
| Volume | 67-68 |
| DOIs | |
| State | Published - Jun 2003 |
| Externally published | Yes |
| Event | Proceedings of the 28th International Conference on MNE - Lugano, Switzerland Duration: Sep 16 2002 → Sep 19 2002 |
Keywords
- Contact lithography
- DNA
- Electrophoresis
- Fluorescence microscopy
- Microchip
- Single molecule
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Electrical and Electronic Engineering