Site-specific recombinational cloning using gateway and in-fusion cloning schemes

Jaehong Park, Andrea L. Throop, Joshua LaBaer

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The comprehensive study of protein structure and function, or proteomics, depends on the obtainability of full-length cDNAs in species-specific expression vectors and subsequent functional analysis of the expressed protein. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence of interest, which differentiates it from classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using the most popular commercial recombinational cloning technologies, Gateway (Life Technologies) and In-Fusion (Clontech).

Original languageEnglish (US)
Pages (from-to)3.20.1-3.20.23
JournalCurrent Protocols in Molecular Biology
Volume2015
DOIs
StatePublished - 2015

Keywords

  • Cre-lox
  • Gateway
  • High-throughput
  • In-fusion
  • Recombinational cloning

ASJC Scopus subject areas

  • Molecular Biology

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