Site-directed mutagenesis of M1 RNA, the RNA subunit of Escherichia coli ribonuclease P. The effects of an addition and small deletions on catalytic function

Nathan P. Lawrence, Sidney Altman

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

One addition mutation and several small deletion mutations have been created in vitro at a unique site in the gene coding for M1 RNA, the RNA subunit of Escherichia coli RNase P. The mutant genes exhibit a wide range of efficiencies in complementing another mutant that is thermosensitive for RNase P function in vivo. The transcripts of the mutated genes cleave a precursor tRNA in vitro with efficiencies that parallel their ability to function in the complementation assay in vivo. The secondary structures in solution of the mutant gene transcripts are shown to be different from the parent molecule by probing the structure of the transcripts with ribonuclease T1. A local region of secondary structure, between nucleotides 275 amd 295, must be maintained for normal function of M1 RNA.

Original languageEnglish (US)
Pages (from-to)163-175
Number of pages13
JournalJournal of molecular biology
Volume191
Issue number2
DOIs
StatePublished - Sep 20 1986
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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