Abstract

Self-folding of an information-carrying polymer into a defined structure is foundational to biology and offers attractive potential as a synthetic strategy. Although multicomponent self-assembly has produced complex synthetic nanostructures, unimolecular folding has seen limited progress.We describe a framework to design and synthesize a single DNA or RNA strand to self-fold into a complex yet unknotted structure that approximates an arbitrary user-prescribed shape. We experimentally construct diverse multikilobase single-stranded structures, including a ∼10, 000-nucleotide (nt) DNA structure and a ∼6000-nt RNA structure. We demonstrate facile replication of the strand in vitro and in living cells. The work here thus establishes unimolecular folding as a general strategy for constructing complex and replicable nucleic acid nanostructures, and expands the design space and material scalability for bottom-up nanotechnology.

Original languageEnglish (US)
Article numberaao2648
JournalScience
Volume358
Issue number6369
DOIs
StatePublished - Dec 15 2017

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Nanostructures
Single-Stranded DNA
Nucleotides
RNA
Nanotechnology
DNA
Nucleic Acids
Polymers
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Han, D., Qi, X., Myhrvold, C., Wang, B., Dai, M., Jiang, S., ... Yin, P. (2017). Single-stranded DNA and RNA origami. Science, 358(6369), [aao2648]. https://doi.org/10.1126/science.aao2648

Single-stranded DNA and RNA origami. / Han, Dongran; Qi, Xiaodong; Myhrvold, Cameron; Wang, Bei; Dai, Mingjie; Jiang, Shuoxing; Bates, Maxwell; Liu, Yan; An, Byoungkwon; Zhang, Fei; Yan, Hao; Yin, Peng.

In: Science, Vol. 358, No. 6369, aao2648, 15.12.2017.

Research output: Contribution to journalArticle

Han, D, Qi, X, Myhrvold, C, Wang, B, Dai, M, Jiang, S, Bates, M, Liu, Y, An, B, Zhang, F, Yan, H & Yin, P 2017, 'Single-stranded DNA and RNA origami', Science, vol. 358, no. 6369, aao2648. https://doi.org/10.1126/science.aao2648
Han D, Qi X, Myhrvold C, Wang B, Dai M, Jiang S et al. Single-stranded DNA and RNA origami. Science. 2017 Dec 15;358(6369). aao2648. https://doi.org/10.1126/science.aao2648
Han, Dongran ; Qi, Xiaodong ; Myhrvold, Cameron ; Wang, Bei ; Dai, Mingjie ; Jiang, Shuoxing ; Bates, Maxwell ; Liu, Yan ; An, Byoungkwon ; Zhang, Fei ; Yan, Hao ; Yin, Peng. / Single-stranded DNA and RNA origami. In: Science. 2017 ; Vol. 358, No. 6369.
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AU - Bates, Maxwell

AU - Liu, Yan

AU - An, Byoungkwon

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