Single-particle imaging without symmetry constraints at an X-ray free-electron laser

Max Rose, Sergey Bobkov, Kartik Ayyer, Ruslan P. Kurta, Dmitry Dzhigaev, Young Yong Kim, Andrew J. Morgan, Chun Hong Yoon, Daniel Westphal, Johan Bielecki, Jonas A. Sellberg, Garth Williams, Filipe R.N.C. Maia, Olexander M. Yefanov, Vyacheslav Ilyin, Adrian P. Mancuso, Henry N. Chapman, Brenda Hogue, Andrew Aquila, Anton Barty & 1 others Ivan A. Vartanyants

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.

    Original languageEnglish (US)
    Pages (from-to)727-736
    Number of pages10
    JournalIUCrJ
    Volume5
    DOIs
    StatePublished - Jan 1 2018

    Fingerprint

    X ray lasers
    Free electron lasers
    viruses
    Viruses
    free electron lasers
    Lasers
    X-Rays
    Electrons
    Imaging techniques
    symmetry
    bacteriophages
    Bacteriophages
    x rays
    Workflow
    Diffraction patterns
    Carrier concentration
    indication
    diffraction patterns
    Diffraction
    Experiments

    Keywords

    • single-particle imaging
    • three-dimensional virus reconstructions
    • XFELs

    ASJC Scopus subject areas

    • Chemistry(all)
    • Biochemistry
    • Materials Science(all)
    • Condensed Matter Physics

    Cite this

    Rose, M., Bobkov, S., Ayyer, K., Kurta, R. P., Dzhigaev, D., Kim, Y. Y., ... Vartanyants, I. A. (2018). Single-particle imaging without symmetry constraints at an X-ray free-electron laser. IUCrJ, 5, 727-736. https://doi.org/10.1107/S205225251801120X

    Single-particle imaging without symmetry constraints at an X-ray free-electron laser. / Rose, Max; Bobkov, Sergey; Ayyer, Kartik; Kurta, Ruslan P.; Dzhigaev, Dmitry; Kim, Young Yong; Morgan, Andrew J.; Yoon, Chun Hong; Westphal, Daniel; Bielecki, Johan; Sellberg, Jonas A.; Williams, Garth; Maia, Filipe R.N.C.; Yefanov, Olexander M.; Ilyin, Vyacheslav; Mancuso, Adrian P.; Chapman, Henry N.; Hogue, Brenda; Aquila, Andrew; Barty, Anton; Vartanyants, Ivan A.

    In: IUCrJ, Vol. 5, 01.01.2018, p. 727-736.

    Research output: Contribution to journalArticle

    Rose, M, Bobkov, S, Ayyer, K, Kurta, RP, Dzhigaev, D, Kim, YY, Morgan, AJ, Yoon, CH, Westphal, D, Bielecki, J, Sellberg, JA, Williams, G, Maia, FRNC, Yefanov, OM, Ilyin, V, Mancuso, AP, Chapman, HN, Hogue, B, Aquila, A, Barty, A & Vartanyants, IA 2018, 'Single-particle imaging without symmetry constraints at an X-ray free-electron laser' IUCrJ, vol. 5, pp. 727-736. https://doi.org/10.1107/S205225251801120X
    Rose M, Bobkov S, Ayyer K, Kurta RP, Dzhigaev D, Kim YY et al. Single-particle imaging without symmetry constraints at an X-ray free-electron laser. IUCrJ. 2018 Jan 1;5:727-736. https://doi.org/10.1107/S205225251801120X
    Rose, Max ; Bobkov, Sergey ; Ayyer, Kartik ; Kurta, Ruslan P. ; Dzhigaev, Dmitry ; Kim, Young Yong ; Morgan, Andrew J. ; Yoon, Chun Hong ; Westphal, Daniel ; Bielecki, Johan ; Sellberg, Jonas A. ; Williams, Garth ; Maia, Filipe R.N.C. ; Yefanov, Olexander M. ; Ilyin, Vyacheslav ; Mancuso, Adrian P. ; Chapman, Henry N. ; Hogue, Brenda ; Aquila, Andrew ; Barty, Anton ; Vartanyants, Ivan A. / Single-particle imaging without symmetry constraints at an X-ray free-electron laser. In: IUCrJ. 2018 ; Vol. 5. pp. 727-736.
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