@article{2beae288c0974d42916f06f3b6d9daf7,
title = "Single-particle imaging without symmetry constraints at an X-ray free-electron laser",
abstract = "The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.",
keywords = "XFELs, single-particle imaging, three-dimensional virus reconstructions",
author = "Max Rose and Sergey Bobkov and Kartik Ayyer and Kurta, {Ruslan P.} and Dmitry Dzhigaev and Kim, {Young Yong} and Morgan, {Andrew J.} and Yoon, {Chun Hong} and Daniel Westphal and Johan Bielecki and Sellberg, {Jonas A.} and Garth Williams and Maia, {Filipe R.N.C.} and Yefanov, {Olexander M.} and Vyacheslav Ilyin and Mancuso, {Adrian P.} and Chapman, {Henry N.} and Brenda Hogue and Andrew Aquila and Anton Barty and Vartanyants, {Ivan A.}",
note = "Funding Information: Portions of this research were carried out at the LCLS at the SLAC National Accelerator Laboratory; use of the LCLS is supported by the US Department of Energy, Office of Science,Office of Basic Energy Sciences (under Contract No. DE-AC02-76SF00515). MR, SB, VI and IAV acknowledge support from the Helmholtz Association{\textquoteright}s Initiative and Networking Fund and the Russian Science Foundation (project No. 18-41-06001). MR acknowledges financial support from the Joachim Herz Foundation Hamburg. MR, KA, YYK, AJM and AB acknowledge support of Program-Oriented Funds of the Helmholtz Association to DESY. JAS and FRNCM acknowledge support from the Swedish Research Council and the Swedish Foundation for Strategic Research. BGH acknowledges support from the US National Science Foundation (NSF) Science and Technology Center BioXFEL Award 1231306. Publisher Copyright: {\textcopyright} 2018 Max Rose et al.",
year = "2018",
doi = "10.1107/S205225251801120X",
language = "English (US)",
volume = "5",
pages = "727--736",
journal = "IUCrJ",
issn = "2052-2525",
publisher = "International Union of Crystallography",
}