Single-molecule recognition imaging microscopy

C. Stroh, H. Wang, R. Bash, B. Ashcroft, J. Nelson, H. Gruber, D. Lohr, Stuart Lindsay, P. Hinterdorfer

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Abstract

Atomic force microscopy is a powerful and widely used imaging technique that can visualize single molecules and follow processes at the single-molecule level both in air and in solution. For maximum usefulness in biological applications, atomic force microscopy needs to be able to identify specific types of molecules in an image, much as fluorescent tags do for optical microscopy. The results presented here demonstrate that the highly specific antibody-antigen interaction can be used to generate single-molecule maps of specific types of molecules in a compositionally complex sample while simultaneously carrying out high-resolution topographic imaging. Because it can identify specific components, the technique can be used to map composition over an image and to detect compositional changes occurring during a process.

Original languageEnglish (US)
Pages (from-to)12503-12507
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number34
DOIs
Publication statusPublished - Aug 24 2004

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ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Stroh, C., Wang, H., Bash, R., Ashcroft, B., Nelson, J., Gruber, H., ... Hinterdorfer, P. (2004). Single-molecule recognition imaging microscopy. Proceedings of the National Academy of Sciences of the United States of America, 101(34), 12503-12507. https://doi.org/10.1073/pnas.0403538101