Recent developments in single molecule force spectroscopy (SMFS) allow direct observation and measurements of forces that hold protein-DNA complexes together. Furthermore, the mechanics of double-stranded (ds) DNA molecules in the presence of small binding ligands can be detected. The results elucidate molecular binding mechanisms and open the way for ultra sensitive and powerful biosensor applications.
- Atomic force microscopy
- Ligand-DNA interaction
- Optical tweezers
- Single molecule force spectroscopy
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology