Single molecule force spectroscopy on ligand-DNA complexes: From molecular binding mechanisms to biosensor applications

Robert Ros, Rainer Eckel, Frank Bartels, Andy Sischka, Birgit Baumgarth, Sven David Wilking, Alfred Pühler, Norbert Sewald, Anke Becker, Dario Anselmetti

Research output: Contribution to journalShort surveypeer-review

27 Scopus citations

Abstract

Recent developments in single molecule force spectroscopy (SMFS) allow direct observation and measurements of forces that hold protein-DNA complexes together. Furthermore, the mechanics of double-stranded (ds) DNA molecules in the presence of small binding ligands can be detected. The results elucidate molecular binding mechanisms and open the way for ultra sensitive and powerful biosensor applications.

Original languageEnglish (US)
Pages (from-to)5-12
Number of pages8
JournalJournal of Biotechnology
Volume112
Issue number1-2
DOIs
StatePublished - Aug 26 2004
Externally publishedYes

Keywords

  • Atomic force microscopy
  • Ligand-DNA interaction
  • Optical tweezers
  • Single molecule force spectroscopy

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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