Single molecule force spectroscopy in biology using the atomic force microscope

Jordanka Zlatanova; Stuart Lindsay; Sanford H. Leuba

doi:10.1016/S0079-6107(00)00014-6

Single molecule force spectroscopy in biology using the atomic force microscope

Jordanka Zlatanova, Stuart Lindsay, Sanford H. Leuba

Research output: Contribution to journal › Review article › peer-review

345 Scopus citations

Abstract

The importance of forces in biology has been recognized for quite a while but only in the past decade have we acquired instrumentation and methodology to directly measure interactive forces at the level of single biological macromolecules and/or their complexes. This review focuses on force measurements performed with the atomic force microscope. A general introduction to the principle of action is followed by review of the types of interactions being studied, describing the main results and discussing the biological implications.

Original language	English (US)
Pages (from-to)	37-61
Number of pages	25
Journal	Progress in Biophysics and Molecular Biology
Volume	74
Issue number	1-2
DOIs	https://doi.org/10.1016/S0079-6107(00)00014-6
State	Published - 2000

Keywords

Atomic force microscopy
DNA stretching
Intermolecular interactions
Intramolecular interactions
Protein unfolding
Single molecule force Spectroscopy

ASJC Scopus subject areas

Biophysics
Molecular Biology

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10.1016/S0079-6107(00)00014-6

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abstract = "The importance of forces in biology has been recognized for quite a while but only in the past decade have we acquired instrumentation and methodology to directly measure interactive forces at the level of single biological macromolecules and/or their complexes. This review focuses on force measurements performed with the atomic force microscope. A general introduction to the principle of action is followed by review of the types of interactions being studied, describing the main results and discussing the biological implications.",

keywords = "Atomic force microscopy, DNA stretching, Intermolecular interactions, Intramolecular interactions, Protein unfolding, Single molecule force Spectroscopy",

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AU - Lindsay, Stuart

AU - Leuba, Sanford H.

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AB - The importance of forces in biology has been recognized for quite a while but only in the past decade have we acquired instrumentation and methodology to directly measure interactive forces at the level of single biological macromolecules and/or their complexes. This review focuses on force measurements performed with the atomic force microscope. A general introduction to the principle of action is followed by review of the types of interactions being studied, describing the main results and discussing the biological implications.

KW - Atomic force microscopy

KW - DNA stretching

KW - Intermolecular interactions

KW - Intramolecular interactions

KW - Protein unfolding

KW - Single molecule force Spectroscopy

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Single molecule force spectroscopy in biology using the atomic force microscope

Abstract

Keywords

ASJC Scopus subject areas

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