Single-molecule fluorescence studies of IDPs and IDRs

Wenwei Zheng; Hoi Sung Chung

doi:10.1016/B978-0-12-816348-1.00004-1

Single-molecule fluorescence studies of IDPs and IDRs

Wenwei Zheng, Hoi Sung Chung

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Within the arsenal of experimental methods in studying intrinsically disordered proteins (IDPs), single-molecule fluorescence methods provide unique long-range distance and dynamics information. In this chapter, we discuss recent progress on using single-molecule fluorescence methods for probing both intra- and intermolecular interactions of IDPs. We focus on several aspects, including models for analyzing experimental data, physical principles that can be obtained, and combinations with other experimental methods for a comprehensive view of the structural properties of IDPs.

Original language	English (US)
Title of host publication	Intrinsically Disordered Proteins
Subtitle of host publication	Dynamics, Binding, and Function
Publisher	Elsevier
Pages	93-136
Number of pages	44
ISBN (Electronic)	9780128163481
ISBN (Print)	9780128167328
DOIs	https://doi.org/10.1016/B978-0-12-816348-1.00004-1
State	Published - Jan 1 2019
Externally published	Yes

Keywords

Fluorescence correlation spectroscopy
Molecule dynamics simulation
Polymer model
Protein dynamics
Single-molecule Förster resonance energy transfer

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/B978-0-12-816348-1.00004-1

Cite this

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abstract = "Within the arsenal of experimental methods in studying intrinsically disordered proteins (IDPs), single-molecule fluorescence methods provide unique long-range distance and dynamics information. In this chapter, we discuss recent progress on using single-molecule fluorescence methods for probing both intra- and intermolecular interactions of IDPs. We focus on several aspects, including models for analyzing experimental data, physical principles that can be obtained, and combinations with other experimental methods for a comprehensive view of the structural properties of IDPs.",

keywords = "Fluorescence correlation spectroscopy, Molecule dynamics simulation, Polymer model, Protein dynamics, Single-molecule F{\"o}rster resonance energy transfer",

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AU - Chung, Hoi Sung

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N2 - Within the arsenal of experimental methods in studying intrinsically disordered proteins (IDPs), single-molecule fluorescence methods provide unique long-range distance and dynamics information. In this chapter, we discuss recent progress on using single-molecule fluorescence methods for probing both intra- and intermolecular interactions of IDPs. We focus on several aspects, including models for analyzing experimental data, physical principles that can be obtained, and combinations with other experimental methods for a comprehensive view of the structural properties of IDPs.

AB - Within the arsenal of experimental methods in studying intrinsically disordered proteins (IDPs), single-molecule fluorescence methods provide unique long-range distance and dynamics information. In this chapter, we discuss recent progress on using single-molecule fluorescence methods for probing both intra- and intermolecular interactions of IDPs. We focus on several aspects, including models for analyzing experimental data, physical principles that can be obtained, and combinations with other experimental methods for a comprehensive view of the structural properties of IDPs.

KW - Fluorescence correlation spectroscopy

KW - Molecule dynamics simulation

KW - Polymer model

KW - Protein dynamics

KW - Single-molecule Förster resonance energy transfer

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M3 - Chapter

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SN - 9780128167328

SP - 93

EP - 136

BT - Intrinsically Disordered Proteins

PB - Elsevier

ER -

Single-molecule fluorescence studies of IDPs and IDRs

Abstract

Keywords

ASJC Scopus subject areas

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