Single-molecule fluorescence studies of IDPs and IDRs

Wenwei Zheng, Hoi Sung Chung

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Within the arsenal of experimental methods in studying intrinsically disordered proteins (IDPs), single-molecule fluorescence methods provide unique long-range distance and dynamics information. In this chapter, we discuss recent progress on using single-molecule fluorescence methods for probing both intra- and intermolecular interactions of IDPs. We focus on several aspects, including models for analyzing experimental data, physical principles that can be obtained, and combinations with other experimental methods for a comprehensive view of the structural properties of IDPs.

Original languageEnglish (US)
Title of host publicationIntrinsically Disordered Proteins
Subtitle of host publicationDynamics, Binding, and Function
PublisherElsevier
Pages93-136
Number of pages44
ISBN (Electronic)9780128163481
ISBN (Print)9780128167328
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Keywords

  • Fluorescence correlation spectroscopy
  • Molecule dynamics simulation
  • Polymer model
  • Protein dynamics
  • Single-molecule Förster resonance energy transfer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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