Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides

Rainer Eckel, Sven David Wilking, Anke Becker, Norbert Sewald, Robert Ros, Dario Anselmetti

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

(Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.

Original languageEnglish (US)
Pages (from-to)3921-3924
Number of pages4
JournalAngewandte Chemie - International Edition
Volume44
Issue number25
DOIs
StatePublished - Jun 20 2005

Keywords

  • Molecular recognition
  • Peptides
  • Point mutations
  • Scanning probe microscopy
  • Single-molecule studies

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides'. Together they form a unique fingerprint.

  • Cite this