Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides

Rainer Eckel, Sven David Wilking, Anke Becker, Norbert Sewald, Robert Ros, Dario Anselmetti

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

(Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.

Original languageEnglish (US)
Pages (from-to)3921-3924
Number of pages4
JournalAngewandte Chemie - International Edition
Volume44
Issue number25
DOIs
StatePublished - Jun 20 2005
Externally publishedYes

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Peptides
DNA
Molecules
Experiments
Transcription
Synthetic Biology

Keywords

  • Molecular recognition
  • Peptides
  • Point mutations
  • Scanning probe microscopy
  • Single-molecule studies

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Single-molecule experiments in synthetic biology : An approach to the affinity ranking of DNA-binding peptides. / Eckel, Rainer; Wilking, Sven David; Becker, Anke; Sewald, Norbert; Ros, Robert; Anselmetti, Dario.

In: Angewandte Chemie - International Edition, Vol. 44, No. 25, 20.06.2005, p. 3921-3924.

Research output: Contribution to journalArticle

Eckel, Rainer ; Wilking, Sven David ; Becker, Anke ; Sewald, Norbert ; Ros, Robert ; Anselmetti, Dario. / Single-molecule experiments in synthetic biology : An approach to the affinity ranking of DNA-binding peptides. In: Angewandte Chemie - International Edition. 2005 ; Vol. 44, No. 25. pp. 3921-3924.
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