Single-molecule detection of DNA via sequence-specific links between F 1-ATPase motors and gold nanorod sensors

Justin York, David Spetzler, Fusheng Xiong, Wayne Frasch

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We report the construction of a novel biosensing nanodevice to detect single, sequence-specific target DNA molecules. Nanodevice assembly occurs through the association of an immobilized F1-ATPase molecular motor and a functionalized gold nanorod via a single 3′,5′-dibiotinylated DNA molecule. Target-dependent 3′,5′-dibiotinylated DNA bridges form by combining ligation and exonucleation reactions (LXR), with a specificity capable of selecting against a single nucleotide polymorphism (SNP). Using dark field microscopy to detect gold nanorods, quantitation of assembled nanodevices is sufficient to distinguish the presence of as few as 1800 DNA bridges from nonspecifically bound nanorods. The rotary mechanism of F1-ATPase can drive gold nanorod rotation when the nanorod is attached via the DNA bridge. Therefore, rotation discriminates fully assembled devices from nonspecifically bound nanorods, resulting in a sensitivity limit of one zeptomole (600 molecules).

Original languageEnglish (US)
Pages (from-to)415-419
Number of pages5
JournalLab on a Chip
Volume8
Issue number3
DOIs
StatePublished - Jan 1 2008

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ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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