Single-cell real-time PCR: Direct process from cells to data

Xu Shi, Liang I. Lin, Szu Yu Chen, Weimin Gao, Shih-Hui Chao, Weiwen Zhang, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents an easily produced, cost-efficient chip-level device which has the ability to perform real-time PCR with single copy sensitivity. In order to integrate the high sensitivity and accuracy of real-time PCR and advantages of a miniaturized device, a simple chip-level device is built. We used this device to perform real-time PCR with single copy sensitivity. The minimum off-chip work will decrease the handling and human error during the whole process which will help to increase the successful rate and sensitivity of the chip.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages362-364
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Droplet real-time PCR
  • Single-cell
  • Single-copy sensitivity

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Shi, X., Lin, L. I., Chen, S. Y., Gao, W., Chao, S-H., Zhang, W., & Meldrum, D. (2011). Single-cell real-time PCR: Direct process from cells to data. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 362-364). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 1).