Single cell metabolic profiling using multiplexed, photo-patterned fluorescence sensor arrays

Ganquan Song; Kuo Chen Wang; Benjamin Ueberroth; Fred Lee; Liqiang Zhang; Fengyu Su; Haixin Zhu; Qian Mei; Shih-Hui Chao; Laimonas Kelbauskas; Yanqing Tian; Hong Wang; Deirdre Meldrum

Single cell metabolic profiling using multiplexed, photo-patterned fluorescence sensor arrays

Ganquan Song, Kuo Chen Wang, Benjamin Ueberroth, Fred Lee, Liqiang Zhang, Fengyu Su, Haixin Zhu, Qian Mei, Shih-Hui Chao, Laimonas Kelbauskas, Yanqing Tian, Hong Wang, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present a multi-step photo-polymerization process for patterning triple fluorescence sensor arrays on fused silica chips. The sensor arrays can hermetically seal with microwell arrays containing single live cells for simultaneous measurements of metabolic parameters, such as oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).

Original language	English (US)
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	884-886
Number of pages	3
ISBN (Print)	9780979806476
State	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014

Other

Other	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country	United States
City	San Antonio
Period	10/26/14 → 10/30/14

Keywords

Metabolic analysis
Multi-step photo-polymerization
Multiparameter
Single cell

ASJC Scopus subject areas

Control and Systems Engineering

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Cite this

Song, G., Wang, K. C., Ueberroth, B., Lee, F., Zhang, L., Su, F., Zhu, H., Mei, Q., Chao, S-H., Kelbauskas, L., Tian, Y., Wang, H., & Meldrum, D. (2014). Single cell metabolic profiling using multiplexed, photo-patterned fluorescence sensor arrays. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 884-886). Chemical and Biological Microsystems Society.

Single cell metabolic profiling using multiplexed, photo-patterned fluorescence sensor arrays. / Song, Ganquan; Wang, Kuo Chen; Ueberroth, Benjamin; Lee, Fred; Zhang, Liqiang; Su, Fengyu; Zhu, Haixin; Mei, Qian; Chao, Shih-Hui; Kelbauskas, Laimonas; Tian, Yanqing; Wang, Hong; Meldrum, Deirdre.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 884-886.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Song, G, Wang, KC, Ueberroth, B, Lee, F, Zhang, L, Su, F, Zhu, H, Mei, Q, Chao, S-H, Kelbauskas, L, Tian, Y, Wang, H & Meldrum, D 2014, Single cell metabolic profiling using multiplexed, photo-patterned fluorescence sensor arrays. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 884-886, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.

Song, Ganquan ; Wang, Kuo Chen ; Ueberroth, Benjamin ; Lee, Fred ; Zhang, Liqiang ; Su, Fengyu ; Zhu, Haixin ; Mei, Qian ; Chao, Shih-Hui ; Kelbauskas, Laimonas ; Tian, Yanqing ; Wang, Hong ; Meldrum, Deirdre. / Single cell metabolic profiling using multiplexed, photo-patterned fluorescence sensor arrays. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 884-886

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abstract = "We present a multi-step photo-polymerization process for patterning triple fluorescence sensor arrays on fused silica chips. The sensor arrays can hermetically seal with microwell arrays containing single live cells for simultaneous measurements of metabolic parameters, such as oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).",

keywords = "Metabolic analysis, Multi-step photo-polymerization, Multiparameter, Single cell",

author = "Ganquan Song and Wang, {Kuo Chen} and Benjamin Ueberroth and Fred Lee and Liqiang Zhang and Fengyu Su and Haixin Zhu and Qian Mei and Shih-Hui Chao and Laimonas Kelbauskas and Yanqing Tian and Hong Wang and Deirdre Meldrum",

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AU - Song, Ganquan

AU - Wang, Kuo Chen

AU - Ueberroth, Benjamin

AU - Lee, Fred

AU - Zhang, Liqiang

AU - Su, Fengyu

AU - Zhu, Haixin

AU - Mei, Qian

AU - Chao, Shih-Hui

AU - Kelbauskas, Laimonas

AU - Tian, Yanqing

AU - Wang, Hong

AU - Meldrum, Deirdre

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AB - We present a multi-step photo-polymerization process for patterning triple fluorescence sensor arrays on fused silica chips. The sensor arrays can hermetically seal with microwell arrays containing single live cells for simultaneous measurements of metabolic parameters, such as oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).

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KW - Multi-step photo-polymerization

KW - Multiparameter

KW - Single cell

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PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

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