Single cell metabolic profiling using multiplexed, photo-patterned fluorescence sensor arrays

Ganquan Song, Kuo Chen Wang, Benjamin Ueberroth, Fred Lee, Liqiang Zhang, Fengyu Su, Haixin Zhu, Qian Mei, Shih-Hui Chao, Laimonas Kelbauskas, Yanqing Tian, Hong Wang, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a multi-step photo-polymerization process for patterning triple fluorescence sensor arrays on fused silica chips. The sensor arrays can hermetically seal with microwell arrays containing single live cells for simultaneous measurements of metabolic parameters, such as oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages884-886
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

    Fingerprint

Keywords

  • Metabolic analysis
  • Multi-step photo-polymerization
  • Multiparameter
  • Single cell

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Song, G., Wang, K. C., Ueberroth, B., Lee, F., Zhang, L., Su, F., Zhu, H., Mei, Q., Chao, S-H., Kelbauskas, L., Tian, Y., Wang, H., & Meldrum, D. (2014). Single cell metabolic profiling using multiplexed, photo-patterned fluorescence sensor arrays. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 884-886). Chemical and Biological Microsystems Society.