Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature

Jan Kern, Roberto Alonso-Mori, Rosalie Tran, Johan Hattne, Richard J. Gildea, Nathaniel Echols, Carina Gloc̈kner, Julia Hellmich, Hartawan Laksmono, Raymond G. Sierra, Benedikt Lassalle-Kaiser, Sergey Koroidov, Alyssa Lampe, Guangye Han, Sheraz Gul, Dörte DiFiore, Despina Milathianaki, Alan R. Fry, Alan Miahnahri, Donald W. SchaferMarc Messerschmidt, M. Marvin Seibert, Jason E. Koglin, Dimosthenis Sokaras, Tsu Chien Weng, Jonas Sellberg, Matthew J. Latimer, Ralf W. Grosse-Kunstleve, Petrus H. Zwart, William E. White, Pieter Glatzel, Paul D. Adams, Michael J. Bogan, Garth J. Williams, Sébastien Boutet, Johannes Messinger, Athina Zouni, Nicholas K. Sauter, Vittal K. Yachandra, Uwe Bergmann, Junko Yano

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S 1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.

Original languageEnglish (US)
Pages (from-to)491-495
Number of pages5
JournalScience
Volume340
Issue number6131
DOIs
StatePublished - Apr 26 2013
Externally publishedYes

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Photosystem II Protein Complex
Spectrum Analysis
X-Rays
Temperature
Light
Radiation
Oxygen
Proteins

ASJC Scopus subject areas

  • General

Cite this

Kern, J., Alonso-Mori, R., Tran, R., Hattne, J., Gildea, R. J., Echols, N., ... Yano, J. (2013). Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature. Science, 340(6131), 491-495. https://doi.org/10.1126/science.1234273

Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature. / Kern, Jan; Alonso-Mori, Roberto; Tran, Rosalie; Hattne, Johan; Gildea, Richard J.; Echols, Nathaniel; Gloc̈kner, Carina; Hellmich, Julia; Laksmono, Hartawan; Sierra, Raymond G.; Lassalle-Kaiser, Benedikt; Koroidov, Sergey; Lampe, Alyssa; Han, Guangye; Gul, Sheraz; DiFiore, Dörte; Milathianaki, Despina; Fry, Alan R.; Miahnahri, Alan; Schafer, Donald W.; Messerschmidt, Marc; Seibert, M. Marvin; Koglin, Jason E.; Sokaras, Dimosthenis; Weng, Tsu Chien; Sellberg, Jonas; Latimer, Matthew J.; Grosse-Kunstleve, Ralf W.; Zwart, Petrus H.; White, William E.; Glatzel, Pieter; Adams, Paul D.; Bogan, Michael J.; Williams, Garth J.; Boutet, Sébastien; Messinger, Johannes; Zouni, Athina; Sauter, Nicholas K.; Yachandra, Vittal K.; Bergmann, Uwe; Yano, Junko.

In: Science, Vol. 340, No. 6131, 26.04.2013, p. 491-495.

Research output: Contribution to journalArticle

Kern, J, Alonso-Mori, R, Tran, R, Hattne, J, Gildea, RJ, Echols, N, Gloc̈kner, C, Hellmich, J, Laksmono, H, Sierra, RG, Lassalle-Kaiser, B, Koroidov, S, Lampe, A, Han, G, Gul, S, DiFiore, D, Milathianaki, D, Fry, AR, Miahnahri, A, Schafer, DW, Messerschmidt, M, Seibert, MM, Koglin, JE, Sokaras, D, Weng, TC, Sellberg, J, Latimer, MJ, Grosse-Kunstleve, RW, Zwart, PH, White, WE, Glatzel, P, Adams, PD, Bogan, MJ, Williams, GJ, Boutet, S, Messinger, J, Zouni, A, Sauter, NK, Yachandra, VK, Bergmann, U & Yano, J 2013, 'Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature', Science, vol. 340, no. 6131, pp. 491-495. https://doi.org/10.1126/science.1234273
Kern J, Alonso-Mori R, Tran R, Hattne J, Gildea RJ, Echols N et al. Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature. Science. 2013 Apr 26;340(6131):491-495. https://doi.org/10.1126/science.1234273
Kern, Jan ; Alonso-Mori, Roberto ; Tran, Rosalie ; Hattne, Johan ; Gildea, Richard J. ; Echols, Nathaniel ; Gloc̈kner, Carina ; Hellmich, Julia ; Laksmono, Hartawan ; Sierra, Raymond G. ; Lassalle-Kaiser, Benedikt ; Koroidov, Sergey ; Lampe, Alyssa ; Han, Guangye ; Gul, Sheraz ; DiFiore, Dörte ; Milathianaki, Despina ; Fry, Alan R. ; Miahnahri, Alan ; Schafer, Donald W. ; Messerschmidt, Marc ; Seibert, M. Marvin ; Koglin, Jason E. ; Sokaras, Dimosthenis ; Weng, Tsu Chien ; Sellberg, Jonas ; Latimer, Matthew J. ; Grosse-Kunstleve, Ralf W. ; Zwart, Petrus H. ; White, William E. ; Glatzel, Pieter ; Adams, Paul D. ; Bogan, Michael J. ; Williams, Garth J. ; Boutet, Sébastien ; Messinger, Johannes ; Zouni, Athina ; Sauter, Nicholas K. ; Yachandra, Vittal K. ; Bergmann, Uwe ; Yano, Junko. / Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature. In: Science. 2013 ; Vol. 340, No. 6131. pp. 491-495.
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AU - Kern, Jan

AU - Alonso-Mori, Roberto

AU - Tran, Rosalie

AU - Hattne, Johan

AU - Gildea, Richard J.

AU - Echols, Nathaniel

AU - Gloc̈kner, Carina

AU - Hellmich, Julia

AU - Laksmono, Hartawan

AU - Sierra, Raymond G.

AU - Lassalle-Kaiser, Benedikt

AU - Koroidov, Sergey

AU - Lampe, Alyssa

AU - Han, Guangye

AU - Gul, Sheraz

AU - DiFiore, Dörte

AU - Milathianaki, Despina

AU - Fry, Alan R.

AU - Miahnahri, Alan

AU - Schafer, Donald W.

AU - Messerschmidt, Marc

AU - Seibert, M. Marvin

AU - Koglin, Jason E.

AU - Sokaras, Dimosthenis

AU - Weng, Tsu Chien

AU - Sellberg, Jonas

AU - Latimer, Matthew J.

AU - Grosse-Kunstleve, Ralf W.

AU - Zwart, Petrus H.

AU - White, William E.

AU - Glatzel, Pieter

AU - Adams, Paul D.

AU - Bogan, Michael J.

AU - Williams, Garth J.

AU - Boutet, Sébastien

AU - Messinger, Johannes

AU - Zouni, Athina

AU - Sauter, Nicholas K.

AU - Yachandra, Vittal K.

AU - Bergmann, Uwe

AU - Yano, Junko

PY - 2013/4/26

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