Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature

Jan Kern, Roberto Alonso-Mori, Rosalie Tran, Johan Hattne, Richard J. Gildea, Nathaniel Echols, Carina Gloc̈kner, Julia Hellmich, Hartawan Laksmono, Raymond G. Sierra, Benedikt Lassalle-Kaiser, Sergey Koroidov, Alyssa Lampe, Guangye Han, Sheraz Gul, Dörte DiFiore, Despina Milathianaki, Alan R. Fry, Alan Miahnahri, Donald W. SchaferMarc Messerschmidt, M. Marvin Seibert, Jason E. Koglin, Dimosthenis Sokaras, Tsu Chien Weng, Jonas Sellberg, Matthew J. Latimer, Ralf W. Grosse-Kunstleve, Petrus H. Zwart, William E. White, Pieter Glatzel, Paul D. Adams, Michael J. Bogan, Garth J. Williams, Sébastien Boutet, Johannes Messinger, Athina Zouni, Nicholas K. Sauter, Vittal K. Yachandra, Uwe Bergmann, Junko Yano

Research output: Contribution to journalArticle

288 Scopus citations

Abstract

Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S 1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.

Original languageEnglish (US)
Pages (from-to)491-495
Number of pages5
JournalScience
Volume340
Issue number6131
DOIs
StatePublished - Apr 26 2013

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature'. Together they form a unique fingerprint.

  • Cite this

    Kern, J., Alonso-Mori, R., Tran, R., Hattne, J., Gildea, R. J., Echols, N., Gloc̈kner, C., Hellmich, J., Laksmono, H., Sierra, R. G., Lassalle-Kaiser, B., Koroidov, S., Lampe, A., Han, G., Gul, S., DiFiore, D., Milathianaki, D., Fry, A. R., Miahnahri, A., ... Yano, J. (2013). Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature. Science, 340(6131), 491-495. https://doi.org/10.1126/science.1234273