Simultaneous detection of multiple contrast agents with photoacoustic spectroscopy

Daniel R. Bauer, Leonardo G. Montilla, Mir Salek, Nathan Allen, Kaushal Rege, Russell S. Witte

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Early detection of cancer is important for survival, and multiple targeted contrast agents can be used for advanced cellular profiling. Photoacoustic (PA) imaging is effective at imaging optically-absorbing contrast agents. In this study, we present simulated and experimental results using spectral decomposition to separate the PA absorption spectrum into components from individual contrast agents. The original co-localized concentration of each agent is then simultaneously reconstructed. Monte Carlo simulations were performed to estimate wavelength-dependent fluences in the sample. In phantom experiments, the concentration of two contrast agents (gold nanorods with different absorption spectra) and a background absorbing dye (India Ink) were reconstructed. Finally, we implemented our algorithm in a mouse after injections of gold nanorods with absorption peaks of 750 and 810 nm. The concentration of each nanoparticle was identified from the endogenous background signal with a signal-to-noise ratio of greater than 25 dB.

Original languageEnglish (US)
Title of host publicationIEEE International Ultrasonics Symposium, IUS
Pages1421-1424
Number of pages4
DOIs
StatePublished - 2012
Event2012 IEEE International Ultrasonics Symposium, IUS 2012 - Dresden, Germany
Duration: Oct 7 2012Oct 10 2012

Other

Other2012 IEEE International Ultrasonics Symposium, IUS 2012
CountryGermany
CityDresden
Period10/7/1210/10/12

Fingerprint

photoacoustic spectroscopy
nanorods
gold
absorption spectra
inks
India
mice
fluence
signal to noise ratios
dyes
cancer
injection
decomposition
nanoparticles
estimates
wavelengths
simulation

Keywords

  • breast cancer
  • gold nanorods
  • molecular imaging
  • optoacoustic
  • spectral decomposition algorithm

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

Cite this

Bauer, D. R., Montilla, L. G., Salek, M., Allen, N., Rege, K., & Witte, R. S. (2012). Simultaneous detection of multiple contrast agents with photoacoustic spectroscopy. In IEEE International Ultrasonics Symposium, IUS (pp. 1421-1424). [6562019] https://doi.org/10.1109/ULTSYM.2012.0355

Simultaneous detection of multiple contrast agents with photoacoustic spectroscopy. / Bauer, Daniel R.; Montilla, Leonardo G.; Salek, Mir; Allen, Nathan; Rege, Kaushal; Witte, Russell S.

IEEE International Ultrasonics Symposium, IUS. 2012. p. 1421-1424 6562019.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bauer, DR, Montilla, LG, Salek, M, Allen, N, Rege, K & Witte, RS 2012, Simultaneous detection of multiple contrast agents with photoacoustic spectroscopy. in IEEE International Ultrasonics Symposium, IUS., 6562019, pp. 1421-1424, 2012 IEEE International Ultrasonics Symposium, IUS 2012, Dresden, Germany, 10/7/12. https://doi.org/10.1109/ULTSYM.2012.0355
Bauer DR, Montilla LG, Salek M, Allen N, Rege K, Witte RS. Simultaneous detection of multiple contrast agents with photoacoustic spectroscopy. In IEEE International Ultrasonics Symposium, IUS. 2012. p. 1421-1424. 6562019 https://doi.org/10.1109/ULTSYM.2012.0355
Bauer, Daniel R. ; Montilla, Leonardo G. ; Salek, Mir ; Allen, Nathan ; Rege, Kaushal ; Witte, Russell S. / Simultaneous detection of multiple contrast agents with photoacoustic spectroscopy. IEEE International Ultrasonics Symposium, IUS. 2012. pp. 1421-1424
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