Abstract

This paper presents the design and experimental results of a novel microchip that examines gene expression of single marine diatoms with a streamlined operation. To our knowledge, it is the first microfluidic device that integrates a streamlined process to analyze individual living diatoms that are protected within the silica frustule structures. This simple chip level device takes advantage of droplet arrays to easily load individual cells into isolated droplets and to perform reverse transcription quantitative polymerase chain reaction (RT-qPCR) to analyze gene expression at the single cell level between different growth conditions. The current version of the chip does not need complicated liquid manipulation and is compatible with commercially available thermocyclers.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1924-1926
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

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Keywords

  • Gene expression
  • RT-qPCR
  • Single-cell

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Shi, X., Gao, W., Chao, S-H., & Meldrum, D. (2014). Simple Rt-QPCR chip for single marine diatom cells. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1924-1926). Chemical and Biological Microsystems Society.