Simple microbiologic detection of Campylobacter pylori

J. Parsonnet, K. Welch, Carolyn Compton, R. Strauss, T. Wang, P. Kelsey, M. J. Ferraro

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A study of 84 gastric biopsies taken from 42 adult patients revealed simple techniques for Gram stain and culture for Campylobacter pylori. In an initial study of 18 biopsies, Gram stains prepared from ground, diluted tissue were all negative for curved, gram-negative rods, whereas 13 of these specimens were positive for C. pylori by culture. The Gram stains for the remaining 66 biopsies were prepared by a rinse-imprint technique. In this group, there were 30 Gram stains positive for organisms resembling C. pylori and 32 positive cultures. By Gram-staining two sites, fundus and antrum, the sensitivity of the Gram stain for identifying a positive specimen increased from 91 to 100%. Gram stain may be the preferred technique for rapid diagnosis. When cultured, C. pylori was recovered most often on modified Thayer-Martin medium incubated microaerophilically at 35°C. The presence of antibiotics in modified Thayer-Martin medium limited upper respiratory flora overgrowth, which was often present on nonselective media.

Original languageEnglish (US)
Pages (from-to)948-949
Number of pages2
JournalJournal of Clinical Microbiology
Volume26
Issue number5
StatePublished - 1988
Externally publishedYes

Fingerprint

Helicobacter pylori
Biopsy
Gram's stain
Stomach
Staining and Labeling
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Parsonnet, J., Welch, K., Compton, C., Strauss, R., Wang, T., Kelsey, P., & Ferraro, M. J. (1988). Simple microbiologic detection of Campylobacter pylori. Journal of Clinical Microbiology, 26(5), 948-949.

Simple microbiologic detection of Campylobacter pylori. / Parsonnet, J.; Welch, K.; Compton, Carolyn; Strauss, R.; Wang, T.; Kelsey, P.; Ferraro, M. J.

In: Journal of Clinical Microbiology, Vol. 26, No. 5, 1988, p. 948-949.

Research output: Contribution to journalArticle

Parsonnet, J, Welch, K, Compton, C, Strauss, R, Wang, T, Kelsey, P & Ferraro, MJ 1988, 'Simple microbiologic detection of Campylobacter pylori', Journal of Clinical Microbiology, vol. 26, no. 5, pp. 948-949.
Parsonnet J, Welch K, Compton C, Strauss R, Wang T, Kelsey P et al. Simple microbiologic detection of Campylobacter pylori. Journal of Clinical Microbiology. 1988;26(5):948-949.
Parsonnet, J. ; Welch, K. ; Compton, Carolyn ; Strauss, R. ; Wang, T. ; Kelsey, P. ; Ferraro, M. J. / Simple microbiologic detection of Campylobacter pylori. In: Journal of Clinical Microbiology. 1988 ; Vol. 26, No. 5. pp. 948-949.
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