Signatures of ecological resource availability in the animal and plant proteomes

James Elser, William F. Fagan, Sankar Subramanian, Sudhir Kumar

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Although substantial and ecologically significant differences in elemental composition are well documented for whole organisms, little is known about whether such differences extend to lower levels of biological organization, such as the elemental composition of major molecules. In a proteome-scale investigation of 9 plant genomes and 9 animal genomes, we find that the nitrogen (N) content of plant proteins is lower than that in animal proteins. Furthermore, protein N content declines with the intensity of gene expression for plants, whereas the N content of animal proteins shows no consistent pattern with expression. Additional analyses indicate that the differences in N content between plant and animal proteomes and in plant proteins as a function of gene expression cannot be attributed to protein size, GC content, gene function, or amino acid properties. These patterns suggest that ecophysiological selection has operated to conserve N in plants via decreased reliance on N-rich amino acids. This inference was supported by an analysis of conserved and variable sites indicating that the N content of plant amino acids coded by variable sites is similar to that of the sites conserved between plant and animal genomes and shows no association with expression level. In contrast, in animals, the N content of amino acids coded by variable sites is significantly higher than that for conserved sites, suggesting relaxation of selective constraints for N usage in the animal lineage. This constitutes the first evidence for an influence of environmental resource availability on proteomes of multicellular organisms.

Original languageEnglish (US)
Pages (from-to)1946-1951
Number of pages6
JournalMolecular Biology and Evolution
Volume23
Issue number10
DOIs
StatePublished - Oct 2006

Fingerprint

Proteome
resource availability
proteome
Animals
Availability
animal
protein
Genes
animals
amino acids
amino acid
Plant Genome
animal proteins
Amino Acids
Plant Proteins
plant proteins
genome
Gene expression
Proteins
gene expression

Keywords

  • Animal
  • Nitrogen
  • Plant
  • Proteome
  • Stoichiometry

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

Cite this

Signatures of ecological resource availability in the animal and plant proteomes. / Elser, James; Fagan, William F.; Subramanian, Sankar; Kumar, Sudhir.

In: Molecular Biology and Evolution, Vol. 23, No. 10, 10.2006, p. 1946-1951.

Research output: Contribution to journalArticle

Elser, James ; Fagan, William F. ; Subramanian, Sankar ; Kumar, Sudhir. / Signatures of ecological resource availability in the animal and plant proteomes. In: Molecular Biology and Evolution. 2006 ; Vol. 23, No. 10. pp. 1946-1951.
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