@article{836299c9da0941aab4bf74a720a6bc7d,
title = "Short term, low dose alpha-ketoglutarate based polymeric nanoparticles with methotrexate reverse rheumatoid arthritis symptoms in mice and modulate T helper cell responses",
abstract = "Activated effector T cells induce pro-inflammatory responses in rheumatoid arthritis (RA) which then lead to inflammation of the joints. In this report, we demonstrate that polymeric nanoparticles with alpha keto-glutarate (aKG) in their polymer backbone (termed as paKG NPs) modulate T cell responses in vitro and in vivo. Impressively, a low dose of only three administrations of methotrexate, a clinically and chronically administered drug for RA, in conjunction with two doses of paKG NPs, reversed arthritis symptoms in collagen-induced arthritis (CIA) mice. This was further followed by significant decreases in pro-inflammatory antigen-specific T helper type 17 (TH17) responses and a significant increase in anti-inflammatory regulatory T cell (TREG) responses when CIA treated splenic cells were isolated and re-exposed to the CIA self-antigen. Overall, this study supports the concurrent and short term, low dose of paKG NPs and methotrexate for the reversal of RA symptoms.",
author = "Mangal, {Joslyn L.} and Sahil Inamdar and Suresh, {Abhirami P.} and Jaggarapu, {Madhan Mohan Chandra Sekhar} and Arezoo Esrafili and Ng, {Nathan D.} and Acharya, {Abhinav P.}",
note = "Funding Information: The authors would like to acknowledge the Flow Cytometry Core, the Regenerative Medicine Imaging Facility for histopathological services, the FEI at Erying Materials Center, and the Department of Animal Care and Technologies at Arizona State University. The authors are also grateful for the support of the National Institute of Health (Grant No. 1R01AR078343-01, 1R01GM144966-01 and 1 R01 AI155907-01). Funding Information: The authors would like to acknowledge the Flow Cytometry Core, the Regenerative Medicine Imaging Facility for histopathological services, the FEI at Erying Materials Center, and the Department of Animal Care and Technologies at Arizona State University. The authors are also grateful for the support of the National Institute of Health (Grant No. 1R01AR078343-01, 1R01GM144966-01 and 1 R01 AI155907-01). Publisher Copyright: {\textcopyright} 2022 The Royal Society of Chemistry.",
year = "2022",
month = sep,
day = "27",
doi = "10.1039/d2bm00415a",
language = "English (US)",
volume = "10",
pages = "6688--6697",
journal = "Biomaterials Science",
issn = "2047-4830",
publisher = "Royal Society of Chemistry",
number = "23",
}