TY - JOUR
T1 - Shock wave model for sputtering biomolecules using massive cluster impacts
AU - Mahoney, John F.
AU - Perel, Julius
AU - Lee, Terry D.
AU - Martino, P. A.
AU - Williams, Peter
N1 - Funding Information:
Thea uthors gratefully acknowledge the financial support of the National Institute of Health for this work (Phase I SBIR grant no. 1 R43 GM45656-01, JFM) (ROI GM40673-03, TDL). We also thank Dr. T. J. Ahrens (California Institute of Technology) for helpful discussions regarding shock wave phenomena. We are also grateful to Dr. B. Kalensher and Dr. E. Parilis for many helpful discussions.
PY - 1992/5
Y1 - 1992/5
N2 - A shock wave model is proposed to explain certain features of recently reported spectra obtained by massive duster impact (MCI) mass spectrometry. It is suggested that clusters that impact glycerol matrices with energies/nucleon in the range 0.01 eV/u < E/N < 1.0 eV/u provide an extremely soft method for sputtering intact biomolecules, Compared to the high energy/nucleon characteristic of atomic or molecular ion primary beams (typically < 50 eV/u), massive cluster primary beams possess much lower energies/nucleon, which are insufficient to cause appreciable ionization and radiation damage of matrix material. Moreover, fragmentation products of parent molecular ions are effectively lower. With these benefits, MCI spectra show lower chemical noise background and enhanced signalto-noise ratios. Rankine-Hugoniot analysis of the shock conditions is used to arrive at an estimate of the heat retained in the collision-affected matrix volume after bombardment by a characteristic cluster. For a cluster collision resulting in a 26.8 GPa shock pressure, by analogy with water data, rapid heating of the shocked volume to 1000 °C or more is plausible. In a beam consisting of clusters distributed in size and charge, an estimate is made for the range of cluster sizes over which hyrodynamic shock wave theory applies.
AB - A shock wave model is proposed to explain certain features of recently reported spectra obtained by massive duster impact (MCI) mass spectrometry. It is suggested that clusters that impact glycerol matrices with energies/nucleon in the range 0.01 eV/u < E/N < 1.0 eV/u provide an extremely soft method for sputtering intact biomolecules, Compared to the high energy/nucleon characteristic of atomic or molecular ion primary beams (typically < 50 eV/u), massive cluster primary beams possess much lower energies/nucleon, which are insufficient to cause appreciable ionization and radiation damage of matrix material. Moreover, fragmentation products of parent molecular ions are effectively lower. With these benefits, MCI spectra show lower chemical noise background and enhanced signalto-noise ratios. Rankine-Hugoniot analysis of the shock conditions is used to arrive at an estimate of the heat retained in the collision-affected matrix volume after bombardment by a characteristic cluster. For a cluster collision resulting in a 26.8 GPa shock pressure, by analogy with water data, rapid heating of the shocked volume to 1000 °C or more is plausible. In a beam consisting of clusters distributed in size and charge, an estimate is made for the range of cluster sizes over which hyrodynamic shock wave theory applies.
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U2 - 10.1016/1044-0305(92)87058-7
DO - 10.1016/1044-0305(92)87058-7
M3 - Article
C2 - 24243041
AN - SCOPUS:0000947046
SN - 1044-0305
VL - 3
SP - 311
EP - 317
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 4
ER -