Shear stress influences spatial variations in vascular Mn-SOD expression

Implication for LDL nitration

Lisong Ai, Mahsa Rouhanizadeh, Joseph C. Wu, Wakako Takabe, Hongyu Yu, Mohammad Alavi, Rongsong Li, Yi Chu, Jordan Miller, Donald D. Heistad, Tzung K. Hsiai

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Fluid shear stress modulates vascular production of endothelial superoxide anion (O2.-) and nitric oxide (.NO). Whether the characteristics of shear stress influence the spatial variations in mitochondrial manganese superoxide dismutase (Mn-SOD) expression in vasculatures is not well defined. We constructed a three-dimensional computational fluid dynamics model simulating spatial variations in shear stress at the arterial bifurcation. In parallel, explants of arterial bifurcations were sectioned from the human left main coronary bifurcation and right coronary arteries for immunohistolocalization of Mn-SOD expression. We demonstrated that Mn-SOD staining was prominent in the pulsatile shear stress (PSS)-exposed and atheroprotective regions, but it was nearly absent in the oscillatory shear stress (OSS)-exposed regions and lateral wall of arterial bifurcation. In cultured bovine aortic endothelial cells, PSS at mean shear stress (τave) of 23 dyn/cm2 upregulated Mn-SOD mRNA expression at a higher level than did OSS at τave = 0.02 dyn/cm2 ± 3.0 dyn·cm-2·s-1 and at 1 Hz (PSS by 11.3 ± 0.4-fold vs. OSS by 5.0 ± 0.5-fold vs. static condition; P < 0.05, n = 4). By liquid chromatography and tandem mass spectrometry, it was found that PSS decreased the extent of low-density lipoprotein (LDL) nitration, whereas OSS increased nitration (P < 0.05, n = 4). In the presence of LDL, treatment with Mn-SOD small interfering RNA increased intracellular nitrotyrosine level (P < 0.5, n = 4), a fingerprint for nitrotyrosine formation. Our findings indicate that shear stress in the atheroprone versus atheroprotective regions regulates spatial variations in mitochondrial Mn-SOD expression with an implication for modulating LDL nitration.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume294
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

Fingerprint

Nitration
LDL Lipoproteins
Superoxide Dismutase
Blood Vessels
Shear stress
Birds
Dermatoglyphics
Hydrodynamics
Tandem Mass Spectrometry
Superoxides
Liquid Chromatography
Small Interfering RNA
Coronary Vessels
Nitric Oxide
Endothelial Cells
Staining and Labeling
Messenger RNA
Liquid chromatography
Endothelial cells
Mass spectrometry

Keywords

  • Low-density lipoprotein
  • Nitric oxide
  • Nitrotyrosine
  • Superoxide anion
  • Superoxide dismutase

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Clinical Biochemistry

Cite this

Shear stress influences spatial variations in vascular Mn-SOD expression : Implication for LDL nitration. / Ai, Lisong; Rouhanizadeh, Mahsa; Wu, Joseph C.; Takabe, Wakako; Yu, Hongyu; Alavi, Mohammad; Li, Rongsong; Chu, Yi; Miller, Jordan; Heistad, Donald D.; Hsiai, Tzung K.

In: American Journal of Physiology - Cell Physiology, Vol. 294, No. 6, 06.2008.

Research output: Contribution to journalArticle

Ai, L, Rouhanizadeh, M, Wu, JC, Takabe, W, Yu, H, Alavi, M, Li, R, Chu, Y, Miller, J, Heistad, DD & Hsiai, TK 2008, 'Shear stress influences spatial variations in vascular Mn-SOD expression: Implication for LDL nitration', American Journal of Physiology - Cell Physiology, vol. 294, no. 6. https://doi.org/10.1152/ajpcell.00518.2007
Ai, Lisong ; Rouhanizadeh, Mahsa ; Wu, Joseph C. ; Takabe, Wakako ; Yu, Hongyu ; Alavi, Mohammad ; Li, Rongsong ; Chu, Yi ; Miller, Jordan ; Heistad, Donald D. ; Hsiai, Tzung K. / Shear stress influences spatial variations in vascular Mn-SOD expression : Implication for LDL nitration. In: American Journal of Physiology - Cell Physiology. 2008 ; Vol. 294, No. 6.
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AU - Yu, Hongyu

AU - Alavi, Mohammad

AU - Li, Rongsong

AU - Chu, Yi

AU - Miller, Jordan

AU - Heistad, Donald D.

AU - Hsiai, Tzung K.

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