Sex determination of ancient human skeletons using DNA

Anne Stone, George R. Milner, Svante Paäbo, Mark Stoneking

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

A method for determining the sex of human skeletons was developed using molecular genetic techniques. The amelogenin gene, found on the X and Y chromosomes, was examined using the polymerase chain reaction (PCR) and a nonradioactive dot blot procedure. DNA was analyzed from 20 modern individuals of known sex and 20 skeletons from an archaeological site in central Illinois dating to A.D. 1300. An independent assessment of the sex of each skeleton was made according to standard osteological methods. The sex of 19 ancient and 20 modern individuals was accurately determined using this molecular genetic technique. Molecular sex determination will be especially useful for juvenile and fragmentary remains when it is difficult, or impossible, to establish an individual's sex fram morphological features.

Original languageEnglish (US)
Pages (from-to)231-238
Number of pages8
JournalAmerican Journal of Physical Anthropology
Volume99
Issue number2
DOIs
StatePublished - Feb 1996
Externally publishedYes

Fingerprint

Skeleton
skeleton
gender
DNA
Genetic Techniques
molecular genetics
Molecular Biology
Amelogenin
Y Chromosome
X Chromosome
Y chromosome
X chromosome
methodology
polymerase chain reaction
Polymerase Chain Reaction
Genes
genes

Keywords

  • Amelogenin
  • Ancient DNA
  • Skeletal Sexing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anthropology

Cite this

Sex determination of ancient human skeletons using DNA. / Stone, Anne; Milner, George R.; Paäbo, Svante; Stoneking, Mark.

In: American Journal of Physical Anthropology, Vol. 99, No. 2, 02.1996, p. 231-238.

Research output: Contribution to journalArticle

Stone, Anne ; Milner, George R. ; Paäbo, Svante ; Stoneking, Mark. / Sex determination of ancient human skeletons using DNA. In: American Journal of Physical Anthropology. 1996 ; Vol. 99, No. 2. pp. 231-238.
@article{b3f64518ba674e8da857b9a2084084c3,
title = "Sex determination of ancient human skeletons using DNA",
abstract = "A method for determining the sex of human skeletons was developed using molecular genetic techniques. The amelogenin gene, found on the X and Y chromosomes, was examined using the polymerase chain reaction (PCR) and a nonradioactive dot blot procedure. DNA was analyzed from 20 modern individuals of known sex and 20 skeletons from an archaeological site in central Illinois dating to A.D. 1300. An independent assessment of the sex of each skeleton was made according to standard osteological methods. The sex of 19 ancient and 20 modern individuals was accurately determined using this molecular genetic technique. Molecular sex determination will be especially useful for juvenile and fragmentary remains when it is difficult, or impossible, to establish an individual's sex fram morphological features.",
keywords = "Amelogenin, Ancient DNA, Skeletal Sexing",
author = "Anne Stone and Milner, {George R.} and Svante Pa{\"a}bo and Mark Stoneking",
year = "1996",
month = "2",
doi = "10.1002/(SICI)1096-8644(199602)99:2<231::AID-AJPA1>3.0.CO;2-1",
language = "English (US)",
volume = "99",
pages = "231--238",
journal = "American Journal of Physical Anthropology",
issn = "0002-9483",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Sex determination of ancient human skeletons using DNA

AU - Stone, Anne

AU - Milner, George R.

AU - Paäbo, Svante

AU - Stoneking, Mark

PY - 1996/2

Y1 - 1996/2

N2 - A method for determining the sex of human skeletons was developed using molecular genetic techniques. The amelogenin gene, found on the X and Y chromosomes, was examined using the polymerase chain reaction (PCR) and a nonradioactive dot blot procedure. DNA was analyzed from 20 modern individuals of known sex and 20 skeletons from an archaeological site in central Illinois dating to A.D. 1300. An independent assessment of the sex of each skeleton was made according to standard osteological methods. The sex of 19 ancient and 20 modern individuals was accurately determined using this molecular genetic technique. Molecular sex determination will be especially useful for juvenile and fragmentary remains when it is difficult, or impossible, to establish an individual's sex fram morphological features.

AB - A method for determining the sex of human skeletons was developed using molecular genetic techniques. The amelogenin gene, found on the X and Y chromosomes, was examined using the polymerase chain reaction (PCR) and a nonradioactive dot blot procedure. DNA was analyzed from 20 modern individuals of known sex and 20 skeletons from an archaeological site in central Illinois dating to A.D. 1300. An independent assessment of the sex of each skeleton was made according to standard osteological methods. The sex of 19 ancient and 20 modern individuals was accurately determined using this molecular genetic technique. Molecular sex determination will be especially useful for juvenile and fragmentary remains when it is difficult, or impossible, to establish an individual's sex fram morphological features.

KW - Amelogenin

KW - Ancient DNA

KW - Skeletal Sexing

UR - http://www.scopus.com/inward/record.url?scp=0030052760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030052760&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-8644(199602)99:2<231::AID-AJPA1>3.0.CO;2-1

DO - 10.1002/(SICI)1096-8644(199602)99:2<231::AID-AJPA1>3.0.CO;2-1

M3 - Article

C2 - 8967324

AN - SCOPUS:0030052760

VL - 99

SP - 231

EP - 238

JO - American Journal of Physical Anthropology

JF - American Journal of Physical Anthropology

SN - 0002-9483

IS - 2

ER -