Severely obese have greater LPS-stimulated TNF-α production than normal weight African-American women

Michael L. Kueht, Brian K. McFarlin, Rebecca Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Obesity is associated with an increase in chronic, low-grade inflammation which has been implicated in the development of type 2 diabetes mellitus and cardiovascular disease. The purpose of this study was to determine whether obesity was associated with an elevation of whole blood lipopolysaccharide (LPS)-stimulated tumor necrosis factor-α (TNF-α) production. African-American women were recruited from a larger study and assigned to one of five groups based on BMI: normal weight (NORM; BMI 20-25, n = 7), overweight (OVER; BMI 25-30, n = 12), class 1 obese (OB1; BMI 30-35, n = 19), class 2 obese (OB2; BMI 35-40, n = 10), or class 3 obese (OB3; BMI >40, n = 17). Body composition was determined via a whole body dual-energy X-ray absorptiometry (DXA) scan. Venous blood samples were collected following an overnight fast (>8 h), and stimulated with five doses of LPS (Salmonella enteriditis): 80, 40, 20, 10, and 5 νg/ml for 24 h in a 37 °C, 5% CO 2 incubator. Following stimulation, TNF-α was measured using enzyme-linked immunosorbent assay. OB3 produced 365% more TNF-α than NORM at an LPS dose of 20 μg/ml (P < 0.05). When maximal TNF-α production was assessed regardless of LPS dose, OB3 produced 230% more than NORM and OVER produced 190% more than NW (P = 0.001). Total and trunk fat mass and BMI were significantly correlated with maximal TNF-α production and LPS = 20 μg/ml. Our findings are consistent with previous reports suggesting a relationship between increased adiposity and inflammatory marker production. This is one of the first studies to focus on African-American women, who have higher rates of obesity.

Original languageEnglish (US)
Pages (from-to)447-451
Number of pages5
JournalObesity
Volume17
Issue number3
DOIs
StatePublished - Mar 2009
Externally publishedYes

Fingerprint

African Americans
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Weights and Measures
Obesity
Incubators
Photon Absorptiometry
Adiposity
Carbon Monoxide
Body Composition
Salmonella
Type 2 Diabetes Mellitus
Cardiovascular Diseases
Enzyme-Linked Immunosorbent Assay
Fats
Inflammation

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Severely obese have greater LPS-stimulated TNF-α production than normal weight African-American women. / Kueht, Michael L.; McFarlin, Brian K.; Lee, Rebecca.

In: Obesity, Vol. 17, No. 3, 03.2009, p. 447-451.

Research output: Contribution to journalArticle

Kueht, Michael L. ; McFarlin, Brian K. ; Lee, Rebecca. / Severely obese have greater LPS-stimulated TNF-α production than normal weight African-American women. In: Obesity. 2009 ; Vol. 17, No. 3. pp. 447-451.
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