TY - JOUR
T1 - Severely obese have greater LPS-stimulated TNF-α production than normal weight African-American women
AU - Kueht, Michael L.
AU - McFarlin, Brian K.
AU - Lee, Rebecca E.
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009/3
Y1 - 2009/3
N2 - Obesity is associated with an increase in chronic, low-grade inflammation which has been implicated in the development of type 2 diabetes mellitus and cardiovascular disease. The purpose of this study was to determine whether obesity was associated with an elevation of whole blood lipopolysaccharide (LPS)-stimulated tumor necrosis factor-α (TNF-α) production. African-American women were recruited from a larger study and assigned to one of five groups based on BMI: normal weight (NORM; BMI 20-25, n = 7), overweight (OVER; BMI 25-30, n = 12), class 1 obese (OB1; BMI 30-35, n = 19), class 2 obese (OB2; BMI 35-40, n = 10), or class 3 obese (OB3; BMI >40, n = 17). Body composition was determined via a whole body dual-energy X-ray absorptiometry (DXA) scan. Venous blood samples were collected following an overnight fast (>8 h), and stimulated with five doses of LPS (Salmonella enteriditis): 80, 40, 20, 10, and 5 νg/ml for 24 h in a 37 °C, 5% CO 2 incubator. Following stimulation, TNF-α was measured using enzyme-linked immunosorbent assay. OB3 produced 365% more TNF-α than NORM at an LPS dose of 20 μg/ml (P < 0.05). When maximal TNF-α production was assessed regardless of LPS dose, OB3 produced 230% more than NORM and OVER produced 190% more than NW (P = 0.001). Total and trunk fat mass and BMI were significantly correlated with maximal TNF-α production and LPS = 20 μg/ml. Our findings are consistent with previous reports suggesting a relationship between increased adiposity and inflammatory marker production. This is one of the first studies to focus on African-American women, who have higher rates of obesity.
AB - Obesity is associated with an increase in chronic, low-grade inflammation which has been implicated in the development of type 2 diabetes mellitus and cardiovascular disease. The purpose of this study was to determine whether obesity was associated with an elevation of whole blood lipopolysaccharide (LPS)-stimulated tumor necrosis factor-α (TNF-α) production. African-American women were recruited from a larger study and assigned to one of five groups based on BMI: normal weight (NORM; BMI 20-25, n = 7), overweight (OVER; BMI 25-30, n = 12), class 1 obese (OB1; BMI 30-35, n = 19), class 2 obese (OB2; BMI 35-40, n = 10), or class 3 obese (OB3; BMI >40, n = 17). Body composition was determined via a whole body dual-energy X-ray absorptiometry (DXA) scan. Venous blood samples were collected following an overnight fast (>8 h), and stimulated with five doses of LPS (Salmonella enteriditis): 80, 40, 20, 10, and 5 νg/ml for 24 h in a 37 °C, 5% CO 2 incubator. Following stimulation, TNF-α was measured using enzyme-linked immunosorbent assay. OB3 produced 365% more TNF-α than NORM at an LPS dose of 20 μg/ml (P < 0.05). When maximal TNF-α production was assessed regardless of LPS dose, OB3 produced 230% more than NORM and OVER produced 190% more than NW (P = 0.001). Total and trunk fat mass and BMI were significantly correlated with maximal TNF-α production and LPS = 20 μg/ml. Our findings are consistent with previous reports suggesting a relationship between increased adiposity and inflammatory marker production. This is one of the first studies to focus on African-American women, who have higher rates of obesity.
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U2 - 10.1038/oby.2008.552
DO - 10.1038/oby.2008.552
M3 - Article
C2 - 19057521
AN - SCOPUS:60849096357
VL - 17
SP - 447
EP - 451
JO - Obesity
JF - Obesity
SN - 1930-7381
IS - 3
ER -