Serotonin depletion attenuates cocaine-seeking behavior in rats

Ly T L Tran-Nguyen, David A. Baker, Katherine A. Grote, Joel Solano, Janet Neisewander

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Rationale: Alterations in serotonin (5-HT) neurotransmission during cocaine withdrawal may be involved in incentive motivation for cocaine. Objective: The present study examined the effects of 5-HT depletion on cocaine- and food-seeking behavior (i.e., non-reinforced operant responding). Methods: Separate groups of rats were trained to lever press for cocaine infusions (0.33 mg/kg/0.1 ml, i.v.) or for food pellets (45-mg Noyes food pellets) on a fixed-ratio one schedule of reinforcement during 14 daily 2-h sessions. Half of each group then received treatment with either saline or the tryptophan hydroxylase inhibitor para-chlorophenylalanine (p-CPA; 100 mg/kg, i.p.) on post-training day 5 and day 6. Twenty-four hours after their last treatment, rats were tested for cocaine- or food-seeking behavior by measuring operant responding in the absence of reinforcement until they reached an extinction criterion of no responses for 30 min. Animals were sacrificed 24 h after testing and brain 5-HT levels in various regions were quantified. Results: In cocaine-trained animals, p-CPA treatment significantly decreased cocaine-seeking behavior and produced a trend toward a decrease in extinction latency relative to saline treatment. In food- trained animals, p-CPA treatment failed to alter any of the behavioral measures during testing, suggesting that p-CPA treatment did not alter the animals' memory or ability to perform an operant response. p-CPA significantly depleted 5-HT by 73-85% in every brain region examined. Conclusion: The results suggest that decreasing 5-HT neurotransmission may decrease incentive motivation for cocaine.

Original languageEnglish (US)
Pages (from-to)60-66
Number of pages7
JournalPsychopharmacology
Volume146
Issue number1
DOIs
StatePublished - 1999

Fingerprint

Cocaine
Serotonin
Motivation
Food
Synaptic Transmission
Fenclonine
Tryptophan Hydroxylase
Reinforcement Schedule
Aptitude
Brain

Keywords

  • Cocaine-seeking behavior
  • Extinction
  • Food-seeking behavior
  • Incentive motivation
  • Para- chlorophenylalanine
  • Serotonin

ASJC Scopus subject areas

  • Pharmacology

Cite this

Serotonin depletion attenuates cocaine-seeking behavior in rats. / Tran-Nguyen, Ly T L; Baker, David A.; Grote, Katherine A.; Solano, Joel; Neisewander, Janet.

In: Psychopharmacology, Vol. 146, No. 1, 1999, p. 60-66.

Research output: Contribution to journalArticle

Tran-Nguyen, LTL, Baker, DA, Grote, KA, Solano, J & Neisewander, J 1999, 'Serotonin depletion attenuates cocaine-seeking behavior in rats', Psychopharmacology, vol. 146, no. 1, pp. 60-66. https://doi.org/10.1007/s002130051088
Tran-Nguyen, Ly T L ; Baker, David A. ; Grote, Katherine A. ; Solano, Joel ; Neisewander, Janet. / Serotonin depletion attenuates cocaine-seeking behavior in rats. In: Psychopharmacology. 1999 ; Vol. 146, No. 1. pp. 60-66.
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