Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser

Hasan Demirci, Raymond G. Sierra, Hartawan Laksmono, Robert L. Shoeman, Sabine Botha, Thomas R.M. Barends, Karol Nass, Ilme Schlichting, R. Bruce Doak, Cornelius Gati, Garth J. Williams, Sébastien Boutet, Marc Messerschmidt, Gerwald Jogl, Albert E. Dahlberg, Steven T. Gregory, Michael J. Bogan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

High-resolution ribosome structures determined by X-ray crystallography have provided important insights into the mechanism of translation. Such studies have thus far relied on large ribosome crystals kept at cryogenic temperatures to reduce radiation damage. Here, the application of serial femtosecond X-ray crystallography (SFX) using an X-ray free-electron laser (XFEL) to obtain diffraction data from ribosome microcrystals in liquid suspension at ambient temperature is described. 30S ribosomal subunit microcrystals diffracted to beyond 6Å resolution, demonstrating the feasibility of using SFX for ribosome structural studies. The ability to collect diffraction data at near-physiological temperatures promises to provide fundamental insights into the structural dynamics of the ribosome and its functional complexes.

Original languageEnglish (US)
Pages (from-to)1066-1069
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume69
Issue number9
DOIs
StatePublished - Sep 1 2013
Externally publishedYes

Fingerprint

X ray lasers
ribosomes
Ribosome Subunits
Microcrystals
X ray crystallography
Free electron lasers
microcrystals
Ribosomes
free electron lasers
X-Ray Diffraction
ambient temperature
Suspensions
Lasers
X-Rays
Electrons
X Ray Crystallography
X ray diffraction
Temperature
crystallography
Liquids

Keywords

  • 30S ribosomal subunit
  • ribosome
  • serial femtosecond X-ray crystallography
  • X-ray free-electron laser

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser. / Demirci, Hasan; Sierra, Raymond G.; Laksmono, Hartawan; Shoeman, Robert L.; Botha, Sabine; Barends, Thomas R.M.; Nass, Karol; Schlichting, Ilme; Doak, R. Bruce; Gati, Cornelius; Williams, Garth J.; Boutet, Sébastien; Messerschmidt, Marc; Jogl, Gerwald; Dahlberg, Albert E.; Gregory, Steven T.; Bogan, Michael J.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 9, 01.09.2013, p. 1066-1069.

Research output: Contribution to journalArticle

Demirci, H, Sierra, RG, Laksmono, H, Shoeman, RL, Botha, S, Barends, TRM, Nass, K, Schlichting, I, Doak, RB, Gati, C, Williams, GJ, Boutet, S, Messerschmidt, M, Jogl, G, Dahlberg, AE, Gregory, ST & Bogan, MJ 2013, 'Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 69, no. 9, pp. 1066-1069. https://doi.org/10.1107/S174430911302099X
Demirci, Hasan ; Sierra, Raymond G. ; Laksmono, Hartawan ; Shoeman, Robert L. ; Botha, Sabine ; Barends, Thomas R.M. ; Nass, Karol ; Schlichting, Ilme ; Doak, R. Bruce ; Gati, Cornelius ; Williams, Garth J. ; Boutet, Sébastien ; Messerschmidt, Marc ; Jogl, Gerwald ; Dahlberg, Albert E. ; Gregory, Steven T. ; Bogan, Michael J. / Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2013 ; Vol. 69, No. 9. pp. 1066-1069.
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