Separation of the bryostatin derivatives by high performance liquid chromatography

Yoshiaki Kamano, Ayano Kotake, Toshihiko Nogawa, Hatsue Hiraide, George Pettit, Cherry L. Herald

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The separation of bryostatin derivatives (26-acetate, 26- metabromobenzoate, 26-ketone, and 13→30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished. Using n-hexane/EtOAc/MeOH/H2O (26:5:1:0.01) as mobile phase for normal phase HPLC and acetonitrile (CH3CN)/H2O system for reverse phase HPLC provided good separation of bryostatin derivatives. In addition, contributions of functional group for eluate order were discussed.

Original languageEnglish (US)
Pages (from-to)399-409
Number of pages11
JournalJournal of Liquid Chromatography and Related Technologies
Volume23
Issue number3
DOIs
StatePublished - 2000

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Bryostatins
High performance liquid chromatography
High Pressure Liquid Chromatography
Derivatives
Epoxy Compounds
Reverse-Phase Chromatography
Ketones
Functional groups
Acetates

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

Separation of the bryostatin derivatives by high performance liquid chromatography. / Kamano, Yoshiaki; Kotake, Ayano; Nogawa, Toshihiko; Hiraide, Hatsue; Pettit, George; Herald, Cherry L.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 23, No. 3, 2000, p. 399-409.

Research output: Contribution to journalArticle

Kamano, Yoshiaki ; Kotake, Ayano ; Nogawa, Toshihiko ; Hiraide, Hatsue ; Pettit, George ; Herald, Cherry L. / Separation of the bryostatin derivatives by high performance liquid chromatography. In: Journal of Liquid Chromatography and Related Technologies. 2000 ; Vol. 23, No. 3. pp. 399-409.
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