Abstract
The separation of bryostatin derivatives (26-acetate, 26- metabromobenzoate, 26-ketone, and 13→30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished. Using n-hexane/EtOAc/MeOH/H2O (26:5:1:0.01) as mobile phase for normal phase HPLC and acetonitrile (CH3CN)/H2O system for reverse phase HPLC provided good separation of bryostatin derivatives. In addition, contributions of functional group for eluate order were discussed.
Original language | English (US) |
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Pages (from-to) | 399-409 |
Number of pages | 11 |
Journal | Journal of Liquid Chromatography and Related Technologies |
Volume | 23 |
Issue number | 3 |
DOIs | |
State | Published - 2000 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Pharmaceutical Science
- Clinical Biochemistry