Separation of the bryostatin derivatives by high performance liquid chromatography

Yoshiaki Kamano, Ayano Kotake, Toshihiko Nogawa, Hatsue Hiraide, George Pettit, Cherry L. Herald

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The separation of bryostatin derivatives (26-acetate, 26- metabromobenzoate, 26-ketone, and 13→30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished. Using n-hexane/EtOAc/MeOH/H2O (26:5:1:0.01) as mobile phase for normal phase HPLC and acetonitrile (CH3CN)/H2O system for reverse phase HPLC provided good separation of bryostatin derivatives. In addition, contributions of functional group for eluate order were discussed.

Original languageEnglish (US)
Pages (from-to)399-409
Number of pages11
JournalJournal of Liquid Chromatography and Related Technologies
Issue number3
StatePublished - 2000

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry


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