Separation of E. coli tRNATyr precursor RNA from E. coli tRNA by hydroxyapatite chromatography

Sidney Altman, B. C. Stark

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Escherichia coli tRNATyr precursor RNA can be easily and quickly separated from unfractionated E. coli tRNA and the RNase P cleavage product of the precursor RNA by hydroxyapatite chromatography. The difference in the elution properties of the tRNATyr precursor RNA and the RNase P cleavage product derived from it or the mature tRNATyr is not due only to their differences in molecular weight. The chromatographic properties of the precursor RNA have been exploited to develop a fast method for enriching E. coli RNA preparations for precursor RNA and also a rapid assay for RNase P.

Original languageEnglish (US)
Pages (from-to)547-554
Number of pages8
JournalAnalytical Biochemistry
Volume59
Issue number2
DOIs
StatePublished - 1974
Externally publishedYes

Fingerprint

RNA, Transfer, Tyr
RNA Precursors
Durapatite
Transfer RNA
Chromatography
Escherichia coli
Ribonuclease P
Assays
Molecular Weight
Molecular weight
RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Separation of E. coli tRNATyr precursor RNA from E. coli tRNA by hydroxyapatite chromatography. / Altman, Sidney; Stark, B. C.

In: Analytical Biochemistry, Vol. 59, No. 2, 1974, p. 547-554.

Research output: Contribution to journalArticle

@article{9173b05f1cb243478e0dc12e6c15fedd,
title = "Separation of E. coli tRNATyr precursor RNA from E. coli tRNA by hydroxyapatite chromatography",
abstract = "Escherichia coli tRNATyr precursor RNA can be easily and quickly separated from unfractionated E. coli tRNA and the RNase P cleavage product of the precursor RNA by hydroxyapatite chromatography. The difference in the elution properties of the tRNATyr precursor RNA and the RNase P cleavage product derived from it or the mature tRNATyr is not due only to their differences in molecular weight. The chromatographic properties of the precursor RNA have been exploited to develop a fast method for enriching E. coli RNA preparations for precursor RNA and also a rapid assay for RNase P.",
author = "Sidney Altman and Stark, {B. C.}",
year = "1974",
doi = "10.1016/0003-2697(74)90308-X",
language = "English (US)",
volume = "59",
pages = "547--554",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Separation of E. coli tRNATyr precursor RNA from E. coli tRNA by hydroxyapatite chromatography

AU - Altman, Sidney

AU - Stark, B. C.

PY - 1974

Y1 - 1974

N2 - Escherichia coli tRNATyr precursor RNA can be easily and quickly separated from unfractionated E. coli tRNA and the RNase P cleavage product of the precursor RNA by hydroxyapatite chromatography. The difference in the elution properties of the tRNATyr precursor RNA and the RNase P cleavage product derived from it or the mature tRNATyr is not due only to their differences in molecular weight. The chromatographic properties of the precursor RNA have been exploited to develop a fast method for enriching E. coli RNA preparations for precursor RNA and also a rapid assay for RNase P.

AB - Escherichia coli tRNATyr precursor RNA can be easily and quickly separated from unfractionated E. coli tRNA and the RNase P cleavage product of the precursor RNA by hydroxyapatite chromatography. The difference in the elution properties of the tRNATyr precursor RNA and the RNase P cleavage product derived from it or the mature tRNATyr is not due only to their differences in molecular weight. The chromatographic properties of the precursor RNA have been exploited to develop a fast method for enriching E. coli RNA preparations for precursor RNA and also a rapid assay for RNase P.

UR - http://www.scopus.com/inward/record.url?scp=0016077162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016077162&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(74)90308-X

DO - 10.1016/0003-2697(74)90308-X

M3 - Article

C2 - 4600322

AN - SCOPUS:0016077162

VL - 59

SP - 547

EP - 554

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -