Separation of bufotalin and cinobufotalin by preparative liquid chromatography

M. Inoue, Y. Kamano, George Pettit, C. R. Smith

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Complete resolution of bufadienolides by traditional column chromatography, or by thin-layer chromatography (TLC), is quite difficult. Separation of various bufadienolide conjugates by high performance liquid chromatography (HPLC) has been described, but not for resolution of the corresponding genins. A preparative HPLC procedure has been developed for resolving the difficultly separable bufotalin (1) and cinobufotalin (2). A Lobar B column packed with LiChromprep Si-60 was used to separate these bufadienolides employing a recycling procedure.

Original languageEnglish (US)
Pages (from-to)2453-2460
Number of pages8
JournalJournal of Liquid Chromatography
Volume10
Issue number11
DOIs
StatePublished - Sep 1 1987

ASJC Scopus subject areas

  • Molecular Medicine

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