Separation of bufotalin and cinobufotalin by preparative liquid chromatography

M. Inoue; Y. Kamano; George Pettit; C. R. Smith

doi:10.1080/01483918708068926

Separation of bufotalin and cinobufotalin by preparative liquid chromatography

M. Inoue, Y. Kamano, George Pettit, C. R. Smith

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7 Scopus citations

Abstract

Complete resolution of bufadienolides by traditional column chromatography, or by thin-layer chromatography (TLC), is quite difficult. Separation of various bufadienolide conjugates by high performance liquid chromatography (HPLC) has been described, but not for resolution of the corresponding genins. A preparative HPLC procedure has been developed for resolving the difficultly separable bufotalin (1) and cinobufotalin (2). A Lobar B column packed with LiChromprep Si-60 was used to separate these bufadienolides employing a recycling procedure.

Original language	English (US)
Pages (from-to)	2453-2460
Number of pages	8
Journal	Journal of Liquid Chromatography
Volume	10
Issue number	11
DOIs	https://doi.org/10.1080/01483918708068926
State	Published - Sep 1 1987

ASJC Scopus subject areas

Molecular Medicine

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abstract = "Complete resolution of bufadienolides by traditional column chromatography, or by thin-layer chromatography (TLC), is quite difficult. Separation of various bufadienolide conjugates by high performance liquid chromatography (HPLC) has been described, but not for resolution of the corresponding genins. A preparative HPLC procedure has been developed for resolving the difficultly separable bufotalin (1) and cinobufotalin (2). A Lobar B column packed with LiChromprep Si-60 was used to separate these bufadienolides employing a recycling procedure.",

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AU - Inoue, M.

AU - Kamano, Y.

AU - Pettit, George

AU - Smith, C. R.

PY - 1987/9/1

Y1 - 1987/9/1

N2 - Complete resolution of bufadienolides by traditional column chromatography, or by thin-layer chromatography (TLC), is quite difficult. Separation of various bufadienolide conjugates by high performance liquid chromatography (HPLC) has been described, but not for resolution of the corresponding genins. A preparative HPLC procedure has been developed for resolving the difficultly separable bufotalin (1) and cinobufotalin (2). A Lobar B column packed with LiChromprep Si-60 was used to separate these bufadienolides employing a recycling procedure.

AB - Complete resolution of bufadienolides by traditional column chromatography, or by thin-layer chromatography (TLC), is quite difficult. Separation of various bufadienolide conjugates by high performance liquid chromatography (HPLC) has been described, but not for resolution of the corresponding genins. A preparative HPLC procedure has been developed for resolving the difficultly separable bufotalin (1) and cinobufotalin (2). A Lobar B column packed with LiChromprep Si-60 was used to separate these bufadienolides employing a recycling procedure.

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Separation of bufotalin and cinobufotalin by preparative liquid chromatography

Abstract

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