Separation of bufotalin and cinobufotalin by preparative liquid chromatography

M. Inoue, Y. Kamano, George Pettit, C. R. Smith

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Complete resolution of bufadienolides by traditional column chromatography, or by thin-layer chromatography (TLC), is quite difficult. Separation of various bufadienolide conjugates by high performance liquid chromatography (HPLC) has been described, but not for resolution of the corresponding genins. A preparative HPLC procedure has been developed for resolving the difficulty separable bufotalin (1) and cinobufotalin (2). A Lobar B column packed with LiChromprep Si-60 was used to separate these bufadienolides employing a recycling procedure.

Original languageEnglish (US)
Pages (from-to)2453-2460
Number of pages8
JournalJournal of Liquid Chromatography
Volume10
Issue number11
StatePublished - 1987

Fingerprint

Bufanolides
Liquid chromatography
High performance liquid chromatography
Liquid Chromatography
High Pressure Liquid Chromatography
Thin layer chromatography
Column chromatography
Recycling
Thin Layer Chromatography
Chromatography
cinobufotalin
bufotalin

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Separation of bufotalin and cinobufotalin by preparative liquid chromatography. / Inoue, M.; Kamano, Y.; Pettit, George; Smith, C. R.

In: Journal of Liquid Chromatography, Vol. 10, No. 11, 1987, p. 2453-2460.

Research output: Contribution to journalArticle

Inoue, M. ; Kamano, Y. ; Pettit, George ; Smith, C. R. / Separation of bufotalin and cinobufotalin by preparative liquid chromatography. In: Journal of Liquid Chromatography. 1987 ; Vol. 10, No. 11. pp. 2453-2460.
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