Separation of biotin labeled proteins from their unlabeled counterparts using immobilized platinum affinity chromatography

Dale Miles, Antonio Garcia

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A stationary phase selective for biotin labeled proteins has been developed by immobilizing platinum(II) ions to a polyacrylamide gel. Bovine serum albumin (BSA) and biotin labeled BSA (BSA-Biotin) have been applied individually to a packed column containing the modified gel. At pH 4.8 using column superficial velocities of 1.0 and 0.25 cm/min respectively, 40% and 73% of the applied BSA-Biotin were bound to the activated gel while no unconjugated BSA was bound. A 1.0 M imidazole-HCl solution at pH 7 was successfully used to elute bound BSA-Biotin, indicating that binding to immobilized Pt(II) is reversible.

Original languageEnglish (US)
Pages (from-to)173-189
Number of pages17
JournalJournal of Chromatography A
Volume702
Issue number1-2
DOIs
StatePublished - May 19 1995

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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