Separation of beta-human chorionic gonadotropin and immunoglobulin G by a miniaturized size exclusion chromatography column

Yongmo Yang, Junseok Chae

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Abstract

This report describes a miniaturized size exclusion chromatography column that effectively preseparates raw samples for medical point-of-care testing (POCT) devices. The minicolumn is constructed of polydimethylsiloxane fabricated on a glass slide. The minicolumn separates 300 ng/ml of beta-human chorionic gonadotropin (Β-hCG) from an immunoglobulin G (IgG)-rich solution (100 μg/ml) in 7.7 min, with 2.23 resolution and 0.018 mm plate height. The complete analyte discrimination shows potential for the sample preparation stage of POCT devices for cancer screening, prognosis, and monitoring.

Original languageEnglish (US)
Article number173902
JournalApplied Physics Letters
Volume94
Issue number17
DOIs
StatePublished - May 11 2009

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ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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