Separation of a-fetoprotein and igg by a miniaturized size exclusion chromatography (SEC) column

Yongmo Yang; Junseok Chae

doi:10.1080/00032710902954391

Separation of a-fetoprotein and igg by a miniaturized size exclusion chromatography (SEC) column

Yongmo Yang, Junseok Chae

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We report a miniature separator for medical microdevices. The separator uses a size-exclusion chromatography (SEC) mini-column in liquid chromatography (LC). The mini-column is fabricated on a glass slide with poly- dimethylsiloxane (PDMS) for structure. Polydivinlybenzene nanobeads (5-20mm in diameter, 100-nm pore size) were used. The SEC mini-column successfully separates a-fetoprotein (AFP) and immunoglobulin G (IgG) at 5 and 9min, respectively, with 0.86 resolution and 0.06-mm plate height. The mini-column shows potential for use in onboard sample preparation in a total analysis system (mTAS) for a point-of-care testing (POCT) device that could be used effectively in cancer screening, diagnosis, and prognosis.

Original language	English (US)
Pages (from-to)	1328-1340
Number of pages	13
Journal	Analytical Letters
Volume	42
Issue number	10
DOIs	https://doi.org/10.1080/00032710902954391
State	Published - 2009

Keywords

A-fetoprotein
Cancer biomarker
Liquid chromatography
MEMS
Separation
Size-exclusion chromatography

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032710902954391

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abstract = "We report a miniature separator for medical microdevices. The separator uses a size-exclusion chromatography (SEC) mini-column in liquid chromatography (LC). The mini-column is fabricated on a glass slide with poly- dimethylsiloxane (PDMS) for structure. Polydivinlybenzene nanobeads (5-20mm in diameter, 100-nm pore size) were used. The SEC mini-column successfully separates a-fetoprotein (AFP) and immunoglobulin G (IgG) at 5 and 9min, respectively, with 0.86 resolution and 0.06-mm plate height. The mini-column shows potential for use in onboard sample preparation in a total analysis system (mTAS) for a point-of-care testing (POCT) device that could be used effectively in cancer screening, diagnosis, and prognosis.",

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Separation of a-fetoprotein and igg by a miniaturized size exclusion chromatography (SEC) column

Abstract

Keywords

ASJC Scopus subject areas

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