Abstract

We report a miniature separator for medical microdevices. The separator uses a size-exclusion chromatography (SEC) mini-column in liquid chromatography (LC). The mini-column is fabricated on a glass slide with poly- dimethylsiloxane (PDMS) for structure. Polydivinlybenzene nanobeads (5-20mm in diameter, 100-nm pore size) were used. The SEC mini-column successfully separates a-fetoprotein (AFP) and immunoglobulin G (IgG) at 5 and 9min, respectively, with 0.86 resolution and 0.06-mm plate height. The mini-column shows potential for use in onboard sample preparation in a total analysis system (mTAS) for a point-of-care testing (POCT) device that could be used effectively in cancer screening, diagnosis, and prognosis.

Original languageEnglish (US)
Pages (from-to)1328-1340
Number of pages13
JournalAnalytical Letters
Volume42
Issue number10
DOIs
StatePublished - Oct 15 2009

Keywords

  • A-fetoprotein
  • Cancer biomarker
  • Liquid chromatography
  • MEMS
  • Separation
  • Size-exclusion chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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